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Living Modified Organism (LMO)
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Yeast modified for the production of Farnesene
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Y1979-Farnasene
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Person:Dr Odair GlavinaPresident of CIBio,Rua James Clerk Maxwell, 315 - Techno ParkCampinas, São Paulo
13069-380, BrazilPhone: 55 19 37839457,Fax: 55 19 37839450,Email: glavina@amyris.com,Website:Related OrganizationAmyris Brasil S.A. (Amyris)Private sector (business and industry)Rua James Clerk Maxwell, 315 - Techno ParkCampinas, São Paulo
13069-380, BrazilPhone: 55 19 37839457,Fax: 55 19 37839450,Email: glavina@amyris.com,Website:
The Y1979 strain of yeast was transformed with the insertion of the coding sequence for Farnesene Synthase to produce beta-farnesene from the fermentation of sugar cane. This is a long chain hydrocarbon that can be used as a biofuel.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45724-5 Organism Saccharomyces cerevisiae (Yeast, YEASX)Fungi
Strain Y1979 derived from PE-2
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-105200-3 Farnesene Synthase gene | Artemisia annua (Artemisia , Sweet Wormwood, Sweet Annie, Sweet Sagewort, Annual Wormwood)Protein coding sequence | Use in industrial applications (Biofuel production)
Modified S. cerevisiae developed through a framework of the modular construction of integration cassettes that are propagated in Escherichia coli.
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- Biofuel
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Saccharomyces cerevisiae strain Y1979 is in a diploid state, resulting in the blocking of sexual reporoduction between the haploid forms α and a. Additionally, genes STE5 and IME1, which are required for sporulation and for early sporulation-specific genes expression, were inactivated in this strain therefore hindering production of haploid ascospores and reducing to insignificant levels the likelihood that the yeast sexually reproduces with other naturally occurring yeasts, including laboratory lineages.
These modifications, coupled with alterations to the mevalonate pathway, make this strain highly dependent on specific conditions for proliferation and maintenance, warranting difficulty for the organism in colonizing the environment by invasion or competition with the natural microbiota.
EN
These modifications, coupled with alterations to the mevalonate pathway, make this strain highly dependent on specific conditions for proliferation and maintenance, warranting difficulty for the organism in colonizing the environment by invasion or competition with the natural microbiota.
- Yeast modified for the production of Farnesene Synthase - CTNBio [ English ]
- Amyris [ English ]
- Microbial engineering for the production of advance biofuels.pdf [ English ]
- Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels [ English ]
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