Record details

Living Modified Organism identity

The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name

Herbicide tolerant maize

Transformation event

DAS40278 x NK603

Unique identifier

DAS-4Ø278-9 x MON-ØØ6Ø3-6

Developer(s)

Record #8035

Dow AgroScience

Description

The stacked maize line DAS-4Ø278-9 x MON-ØØ6Ø3-6 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of the EPSPS and Aryloxyalkanoate dioxygenase genes. The expression of these genes are expected to confer tolerance glyphosate herbicide and 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #104814

DAS-4Ø278-9 - Enlist™ Maize

Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate

Show detection method(s)

Record #14776

MON-ØØ6Ø3-6 - Roundup Ready™ maize

Resistance to herbicides - Glyphosate

Show detection method(s)

Characteristics of the transformation process

Vector

pDAS1740 and PV-ZMGT32

Techniques used for the modification

Cross breeding

Genetic elements construct

RB7 matrix attachment region

#104795

1.17 Kb

Ubiquitin gene promoter

#100362

1.99 Kb

Aryloxyalkanoate dioxygenase gene

#104812

0.89 Kb

Per5 3' Untranslated Region

#104813

0.37 Kb

RB7 matrix attachment region

#104795

1.17 Kb

Rice actin 1 gene promoter

#100364

0.80 Kb

Rice actin 1, intron

#100355

0.60 Kb

Chloroplast transit peptide 2

#100365

0.20 Kb

5-enolpyruvylshikimate-3-phosphate synthase gene

#14979

1.40 Kb

Nopaline Synthase Gene Terminator

#100269

0.30 Kb

CaMV Enhanced 35S promoter

#100366

0.60 Kb

Hsp70 intron

#100359

0.80 Kb

Chloroplast transit peptide 2

#100365

0.20 Kb

5-enolpyruvylshikimate-3-phosphate synthase gene

#14979

1.40 Kb

Nopaline Synthase Gene Terminator

#100269

0.30 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from NK603 vector PV-ZMGT32
Parental organism NK603 contributed a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

DNA insert from DAS40278 vector pDAS1740
DAS-40278-9 corn was modified with the insertion of a plant optimised coding sequence for aryloxyalkanoate dioxygenase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to herbicides
- Glyphosate
Tolerance to 2,4-Dichlorophenoxyacetic acid
Tolerance to aryloxyphenoxypropionate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

DAS-40278-9 - EU Database of Reference Methods for GMO Analysis

MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

MON-ØØ6Ø3-6 - CropLife International Detection Methods Database

Additional Information

Other relevant website address or attached documents

DAS-4Ø278-9 x MON-ØØ6Ø3-6 - ISAAA