Record details

Living Modified Organism identity

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LMO name

Papaya modified for resistance to Papaya Ringspot Virus

Transformation event

X17-2

Unique identifier

UFL-X17CP-9

Developer(s)

Record #106311

Michael J. Davis

Professor, Institute of Food and Agricultural Sciences

Citrus Research and Education Center

University of Florida

700 Experiment Station Road

Lake Alfred, Florida

United States of America, 33850-1299

Description

Transgenic papaya modified to express the coat protein (CP) gene of Hawaiian PRSV isolate HA5-1 thereby conferring resistance to the Papaya Ringspot Virus.

NOTE: This LMO was formerly referred to with the UID UFL-X17CP-6

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12085

Carica papaya - Papaya, Pawpaw, Papaw, CARPA

Characteristics of the transformation process

Vector

pBI121fs

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Nopaline Synthase Gene Promoter

#100270

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

CaMV 35S promoter

#100287

0.00 Kb

PRSV coat protein

#15026

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

A uidA leader sequence from E.Coli is inserted after the CaMV 35S promoter to enhance the expression of PRSV CP in papaya.

The developer reports that the insert in line X17-2 has persisted as a single copy through six generations of progeny following the initial transformation and that no vector backbone sequence is present, as determined by Southern blot. The developer also notes that PCR analysis of the 5th generation progeny of the original transformant reveals the elimination of the frame shift in the prsv-cp gene, and that low level expression of PRSV-CP is detectable by Western blot (FDA).

LMO characteristics

Modified traits

Resistance to diseases and pests
- Viruses
  - Papaya ringspot virus (PRV)

Common use(s)

Food

Additional Information

Other relevant website address or attached documents

Papaya ringspot virus-resistant papaya line X17-2 - FDA

X17-2 - ISAAA

X17-2 - OECD