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Living Modified Organism
(LMO)
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Barley modified for tolerance to drought and nutrient deficiencies
EN
pEXP:CKX1
No
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Organization:Palacky University in Olomouc ()Academic or research institute, Faculty of ScienceKrizkovskeho 8Olomouc,
771 47, Czech RepublicPhone: +420 585 634 060,Fax: +420 585 634 002,Email: dekanat.prf@upol.cz,Website: http://www.prf.upol.cz/en/,
The pEXP:CKX1 barley line has been genetically modified by inserting the Arabidopsis thaliana cytokinin dehydrogenase 1 gene under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice
The Arabidopsis cytokinin oxidase/dehydrogenase 1 enzyme (CKX1) catalyzes the irreversible degradation of the hormone cytokinin in a single enzymatic step by oxidative side chain cleavage causing ectopic depletion of cytokinins in roots. The modification therefore results in the irreversible degradation of the cytokinin hormone that leads to enhanced root meristem proliferation and therefore enhanced root system development which might render the modified plant more tolerant to drought and nutrient deficiencies in the soil.
In addition, the transgenic line has also integrated the hpt selection gene coding for hygromycin phosphotransferase that confers resistance to the antibiotic Hygromycin B. This antibiotic resistance has been used in in vitro culture to screen for genetically modified cells and plants.
EN
The Arabidopsis cytokinin oxidase/dehydrogenase 1 enzyme (CKX1) catalyzes the irreversible degradation of the hormone cytokinin in a single enzymatic step by oxidative side chain cleavage causing ectopic depletion of cytokinins in roots. The modification therefore results in the irreversible degradation of the cytokinin hormone that leads to enhanced root meristem proliferation and therefore enhanced root system development which might render the modified plant more tolerant to drought and nutrient deficiencies in the soil.
In addition, the transgenic line has also integrated the hpt selection gene coding for hygromycin phosphotransferase that confers resistance to the antibiotic Hygromycin B. This antibiotic resistance has been used in in vitro culture to screen for genetically modified cells and plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
cv. Golden Promise
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Barley modified for tolerance to drought and nutrient deficiencies| Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
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Barley modified for tolerance to drought and nutrient deficiencies| Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
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Barley modified for tolerance to drought and nutrient deficiencies| Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
pEXP:CKX1
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-108701-2 Cytokinin dehydrogenase 1 gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Tolerance to abiotic stress (Drought)
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BCH-GENE-SCBD-108705-1 LOC_Os04g11040.1 promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
The transgenic line shows an up to 8-fold higher CKX enzyme activity in roots than the wild type. In contrast, CKX enzyme activity is similar to the control plants in leaves.
The cytokinin dehydrogenase 1 gene is under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice.
The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays polyubiquitin gene.
EN
The cytokinin dehydrogenase 1 gene is under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice.
The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays polyubiquitin gene.
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- Research
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EN
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