Barley modified for tolerance to drought and nutrient deficiencies | BCH-LMO-SCBD-108708 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 02 Sep 2015 last updated: 10 Sep 2015
Living Modified Organism identity
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Barley modified for tolerance to drought and nutrient deficiencies
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pEXP:CKX2
The pEXP:CKX2  barley line has been genetically modified by inserting the Arabidopsis thaliana cytokinin dehydrogenase 2 gene under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice

Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, which are chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants. An important trait regulated by cytokinin is the size of the root system.

A reduced cytokinin status in plants (including barley) causes an enhanced root system which might render plants more tolerant to drought and nutrient deficiencies in the soil. Crop yield is often limited by the availability of water and soil-derived mineral nutrients. A larger root system may enable plants to gain access to more water and nutrients and in this way to cope with adverse environmental conditions.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
Characteristics of the modification process
pEXP:CKX2
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The transgenic line shows an up to 8-fold higher CKX enzyme activity in roots than the wild type. In contrast, CKX enzyme activity is similar to the control plants in leaves.

The cytokinin dehydrogenase 2 gene is under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice.

The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays polyubiquitin gene.
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LMO characteristics
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  • Research
Detection method(s)
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Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 3