Barley modified for increased fungal resistance | BCH-LMO-SCBD-108905 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-108905-1   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
last updated: 30 Sep 2015
Living Modified Organism identity
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Barley modified for increased fungal resistance
EN
pYW210-9-(4001-4360)
No
Barley was modified with the insertion of a synthetic form of endochitinase 42 from Trichoderma harzianum with the aim of increasing resistance to root rot caused by Rhizoctonia species through the hydrolyses of  N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrinsis, which are major structural components of the Rhizoctonia species cell wall.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety: Golden Promise
EN
Characteristics of the modification process
pYW210
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Two gene cassettes are integrated into the transgenic barley line pYW210-9-(4001-4360).

Within the first cassette the expression of the endochitinase gene cThEn42(GC) is regulated by the ubi-1 promoter  together with an exon and an intron of the ubi-1 gene (ubiquitin gene) from Zea mays (maize) and the nopaline synthase gene terminator T-nos from Agrobacterium tumefaciens. Secretion of the synthesized protein to the apoplast is allowed by the signal peptide of HvChi33 (33 kDa endochitinase from Hordeum vulgare L. (barley)) that is located upstream of cThEn42(GC) in the gene cassette. The nucleotide sequence of cThEn42 from the donor organism Trichoderma harzianum was codon optimized (65% GC content) to ensure the expression of the fungal gene in barley.

The second gene cassette comprises the P-ubiZM1, the phosphinothricin acetyltransferase gene from Streptomyces hygroscopicus and T-nos and is used as a selection marker.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
Based on transcriptome and metabolome analysis Kogel et al. (2010) reported no statistically significant differences in resistance towards root rot between the transgenic plant and its wild-type counterpart.
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1