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Living Modified Organism
(LMO)
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Barley modified for increased fungal resistance
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pYW210-9-(4001-4360)
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Person:Justus-Liebig-Universität Gießen, Institut für Phytopathologie und Angewandte ZoologieHeinrich-Buff-Ring 26-32Gießen, Hessen
35392, GermanyPhone: + 49 641 99-37490,Fax: + 49 641 99-37499,Related OrganizationJustus-Liebig-Universität Gießen ()Academic or research instituteHeinrich-Buff-Ring 26-32Gießen, Hessen
35392, GermanyPhone: + 49 641 99-37490,Fax: + 49 641 99-37499,
Barley was modified with the insertion of a synthetic form of endochitinase 42 from Trichoderma harzianum with the aim of increasing resistance to root rot caused by Rhizoctonia species through the hydrolyses of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrinsis, which are major structural components of the Rhizoctonia species cell wall.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
Variety: Golden Promise
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pYW210
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-108904-1 Chitinase 33 transit peptide | Hordeum vulgare (Barley, HORVU)Transit signal
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-108903-1 Endochitinase 42 coding sequence | Trichoderma harzianum (TRIHA)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
Two gene cassettes are integrated into the transgenic barley line pYW210-9-(4001-4360).
Within the first cassette the expression of the endochitinase gene cThEn42(GC) is regulated by the ubi-1 promoter together with an exon and an intron of the ubi-1 gene (ubiquitin gene) from Zea mays (maize) and the nopaline synthase gene terminator T-nos from Agrobacterium tumefaciens. Secretion of the synthesized protein to the apoplast is allowed by the signal peptide of HvChi33 (33 kDa endochitinase from Hordeum vulgare L. (barley)) that is located upstream of cThEn42(GC) in the gene cassette. The nucleotide sequence of cThEn42 from the donor organism Trichoderma harzianum was codon optimized (65% GC content) to ensure the expression of the fungal gene in barley.
The second gene cassette comprises the P-ubiZM1, the phosphinothricin acetyltransferase gene from Streptomyces hygroscopicus and T-nos and is used as a selection marker.
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Within the first cassette the expression of the endochitinase gene cThEn42(GC) is regulated by the ubi-1 promoter together with an exon and an intron of the ubi-1 gene (ubiquitin gene) from Zea mays (maize) and the nopaline synthase gene terminator T-nos from Agrobacterium tumefaciens. Secretion of the synthesized protein to the apoplast is allowed by the signal peptide of HvChi33 (33 kDa endochitinase from Hordeum vulgare L. (barley)) that is located upstream of cThEn42(GC) in the gene cassette. The nucleotide sequence of cThEn42 from the donor organism Trichoderma harzianum was codon optimized (65% GC content) to ensure the expression of the fungal gene in barley.
The second gene cassette comprises the P-ubiZM1, the phosphinothricin acetyltransferase gene from Streptomyces hygroscopicus and T-nos and is used as a selection marker.
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- Research
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Based on transcriptome and metabolome analysis Kogel et al. (2010) reported no statistically significant differences in resistance towards root rot between the transgenic plant and its wild-type counterpart.
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