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Modified Organism
Rubber tree modified to express Human Protamine 1
Record information and status
Record ID
Date of creation
2015-09-18 09:41 UTC (anita@nre.gov.my)
Date of last update
2015-10-09 16:49 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-09 16:49 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Rubber tree modified to express Human Protamine 1
Transformation event
Dr. Sunderasan Elumalai
Research Officer
Genetic Transformation and Tissue Culture Programme, Production Advancement Division
Malaysian Rubber Board (LGM)
Genetic Transformation and Tissue Culture Programme,
Production Advancement Division,
Malaysian Rubber Board,

Sungai Buloh, Selangor
Malaysia, 47000
Hevea brasiliensis was modified to express the human protamine 1 (HP1), which is a therapeutic protein which is commonly used to neutralize the anticoagulant effects of heparin during cardiovascular surgery. Expression of the HP1 gene in Hevea brasiliensis allows for the commercial production of this protein for pharmaceutical purposes.

Hevea expressing HP1 also harbours nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hevea brasiliensis - Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR
Point of collection or acquisition of the recipient organism
Clone GL1
Characteristics of the transformation process
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
Hevien gene promoter
0.00 Kb
Protamine 1 coding sequence
0.00 Kb
Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
0.00 Kb
Neomycin Phosphotransferase II
0.00 Kb
Transcript 7 gene 3' untranslated region
0.00 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The four vector constructs were synthesized each containing a variable length of the heaven gene promoter (pGPTV-HP1-HevP1, pGPTV-HP1-HevP2, pGPTV-HP1-HevP3, and pGPTV-HP1-HevP4) each of which was placed upstream of the protamine 1 coding sequence. 

The HP1 and nptII genetic elements were placed in an antisense orientation relative to each other within the plasmid.

The inserted genetic material is the region between the left boarder and the right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
LMO characteristics
Modified traits
  • Production of medical or pharmaceutical compounds (human or animal)
Common use(s)
  • Pharmaceutical
  • Research
Additional Information

Records referencing this document (2)
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record