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Rubber tree modified to express Human Protamine 1
Dr. Sunderasan Elumalai
Genetic Transformation and Tissue Culture Programme, Production Advancement Division
Malaysian Rubber Board (LGM)
Genetic Transformation and Tissue Culture Programme,
Production Advancement Division,
Malaysian Rubber Board,
Sungai Buloh, Selangor
Hevea brasiliensis was modified to express the human
protamine 1 (HP1), which is a therapeutic protein which is commonly
used to neutralize the anticoagulant effects of heparin during
cardiovascular surgery. Expression of the HP1 gene in Hevea
brasiliensis allows for the commercial production of this
protein for pharmaceutical purposes.
Hevea expressing HP1 also harbours nptII gene that confers
resistance to kanamycin, which was incorporated in the growth media
to select transformed callus after co-cultivation with
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hevea brasiliensis - Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR
- Agrobacterium-mediated DNA transfer
Protamine 1 coding sequence
Nopaline Synthase Gene Terminator
Nopaline Synthase Gene Promoter
Neomycin Phosphotransferase II
Transcript 7 gene 3' untranslated region
The four vector constructs were synthesized each containing a
variable length of the heaven gene promoter (pGPTV-HP1-HevP1,
pGPTV-HP1-HevP2, pGPTV-HP1-HevP3, and pGPTV-HP1-HevP4) each of
which was placed upstream of the protamine 1 coding sequence.
The HP1 and nptII genetic elements were placed in an antisense
orientation relative to each other within the plasmid.
The inserted genetic material is the region between the left
boarder and the right boarder (T-DNA) from the Ti plasmid of
Agrobacterium tumefaciens harbouring the above described gene
- Production of medical or pharmaceutical compounds (human or animal)