Record details

Living Modified Organism identity

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LMO name

Rubber tree modified to express Human Protamine 1

Transformation event

HT4

Developer(s)

Record #108755

Dr. Sunderasan Elumalai

Research Officer

Genetic Transformation and Tissue Culture Programme, Production Advancement Division

Malaysian Rubber Board (LGM)

Genetic Transformation and Tissue Culture Programme,
Production Advancement Division,
Malaysian Rubber Board,

Sungai Buloh, Selangor

Malaysia, 47000

Phone:	+603-61459599
Fax:	+603-61565251
Email:	sunderasan@lgm.gov.my

Description

Hevea brasiliensis was modified to express the human protamine 1 (HP1), which is a therapeutic protein which is commonly used to neutralize the anticoagulant effects of heparin during cardiovascular surgery. Expression of the HP1 gene in Hevea brasiliensis allows for the commercial production of this protein for pharmaceutical purposes.

Hevea expressing HP1 also harbours nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #108280

Hevea brasiliensis - Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR

Point of collection or acquisition of the recipient organism

Clone GL1

Characteristics of the transformation process

Vector

pGPTV-HP1-HevP

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Hevien gene promoter

#108907

0.00 Kb

Protamine 1 coding sequence

#108283

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Nopaline Synthase Gene Promoter

#100270

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

Transcript 7 gene 3' untranslated region

#103067

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The four vector constructs were synthesized each containing a variable length of the heaven gene promoter (pGPTV-HP1-HevP1, pGPTV-HP1-HevP2, pGPTV-HP1-HevP3, and pGPTV-HP1-HevP4) each of which was placed upstream of the protamine 1 coding sequence.

The HP1 and nptII genetic elements were placed in an antisense orientation relative to each other within the plasmid.

The inserted genetic material is the region between the left boarder and the right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.

LMO characteristics

Modified traits

Production of medical or pharmaceutical compounds (human or animal)

Common use(s)

Pharmaceutical
Research

Additional Information

Other relevant website address or attached documents

Cloning of Human Protamine 1 (HP1) and Human Atrial Natriuretic Factor (HANF) cDNAs