SPS-ØØG11-9 - Innate™ G Potato | BCH-LMO-SCBD-109059 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 19 Oct 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Innate™ G Potato
EN
G11
SPS-ØØG11-9
  • - Organization: J.R. Simplot Company () | BCH-CON-SCBD-106427-1
    Organization
    J.R. Simplot Company ()
    Private sector (business and industry)
    5369 West Irving Street
    Boise, ID
    83706, United States of America
    Phone: +1 (208) 780-6066,
    Fax:
    Email:
    Website:
Simplot Innate™ Potato is genetically engineered to silence genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Var. G
EN
Characteristics of the modification process
pSIM1278
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-106420-1 ADP glucose pyrophosphorylase gene promoter | (Potato)
    Promoter
  • BCH-GENE-SCBD-106421-1 Asparagine synthetase-1 gene | (Potato)
    Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
  • BCH-GENE-SCBD-106424-3 Polyphenol oxidase 5 gene | (Earth balls, SCLVE)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | (Potato)
    Promoter
This pSIM1278 transformation vector contained a DNA insert comprising of two expression cassettes.

The first cassette comprises fragments of both the asparagine synthetase-1 gene (Asn1) and the polyphenol oxidase-5 gene (Ppo5), arranged as inverted repeats between the Agp promoter of the ADP  glucose pyrophosphorylase gene (Agp) and the Gbss promoter of the granule-bound starch synthase gene (Gbss) and results in silencing of both the Ppo5 and Asn1 genes.

The second cassette is comprised of fragments of the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL), operably linked to the same Agp and Gbss promoters as the first cassette. The function of the second cassette is to silence the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL).

Southern blot analysis indicated that event G11 contains a single DNA insert comprising the sequences needed for expression of the Asn1/Ppo5 gene silencing cassette, and no vector backbone in the LMO.
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
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