Chimeric wheat gliadin | BCH-GENE-SCBD-109212 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

last updated: 10 Nov 2015
General information
Chimeric wheat gliadin
EN
IR-Gli-SYNTH
EN
Double-stranded RNA
Yes
Additional Information
Two fragments of 170 and 191 bp, corresponding to the most conserved regions from, respectively, α- and ω-gliadins, were selected for use as interfering RNA (IR) fragments. These fragments were amplified from bread wheat, assembled to obtain the full chimeric 361-bp ω/α fragment, cloned into the recombination sites of pCR8/GW/TOPO vector (Invitrogen), and recombined to provide the final pDhp-ω/α vector.
EN
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