The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Pea modified for alpha-amylase expression
Institut für Pflanzengenetik und Kulturpflanzenforschung
Genetically modified pea plants in which the bacterial
alpha-amylase gene is present in homozygous condition do not show
any significant changes in configuration and general appearance.
Nevertheless, it cannot be excluded that the effects of the
recombinant alpha-amylase on the plant will become noticeable under
field conditions. The recombinant alpha-amylase is located in
protein bodies. However, the endogenous seed starch, the main
constituent of pea seeds (approx. 70%), is assumed to be degraded
also at suboptimal temperatures, resulting in the formation of
mainly maltose, maltotriose and alpha-dextrin. The partial change
in the carbohydrate composition may, amongst other things, change
the osmotic value of the seeds and increase their frost resistance.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Unknown seed protein-promoter - Vicia faba - Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean
Alpha-amylase gene - Bacillus licheniformis - BACLI
Changes in quality and/or metabolite content - Carbohydrates
Octopine Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium
Phosphinothricin N-acetyltransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to herbicides - Glufosinate
Transcript 7 gene 3' untranslated region - Agrobacterium tumefaciens - Agrobacterium
Beta-glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
The genetically modified pea plants contain 1800 bp of the 1948-bp
AmyLi sequence (alpha-amylase gene) from Bacillus licheniformis
that is controlled by the seed-specific USP promoter of broad beans
(Vicia faba) and the terminator sequence of the ocs gene from A.
In the genetically modified plants, the bar gene is controlled by
the promoter of the nos gene and the terminator sequence of the g7
gene from A. tumefaciens. It was used for the selec-tion of
transformed plant cells.
The uidA gene that codes for the enzyme beta-glucuronidase is
promoterless and contains the nopaline synthase termination region
of A. tumefaciens. It is a constituent of the Ti region of the
transformation vector pGPTV-BAR. The uidA gene is not expected to
be expressed in the genetically modified pea plants.
- Changes in quality and/or metabolite content
- Resistance to herbicides