MON-87751-7 - Insect resistant soybean | BCH-LMO-SCBD-110062 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 08 Apr 2016
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant soybean
Soybean was modified with the insertion of the Cry1A.105 and Cry2Ab2 proteins which provide protection from feeding damage caused by targeted lepidopteran pests, such as primary target pests velvetbean caterpillar (Anticarcia gemmatalis), soybean looper (Pseudoplusia includens), soybean anxil borer (Epinotia aporema), and sunflower looper (Rachiplusia nu).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
1.722 kb
0.263 kb
3.533 kb
0.399 kb
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
PV-GMIR13196 contains two separate T-DNAs that are each delineated by left and right border regions. The first T-DNA, designated as T-DNA I, contains the crylA.105 and cry2Ab2 expression cassettes, with genetic elements as indicated above.

The second T-DNA, designated as T-DNA II, contains marker genes that allow for simplified selection of transformed tissue. During transformation, both T-DNAs were inserted into the soybean genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate those plants that contain the crylA.105 and cry2Ab2 expression cassettes and do not contain the marker gene expression cassettes.

Characterization of the DNA insert in MON 87751 was conducted using a combination of sequencing, PCR, and bioinformatics. The results of this characterization demonstrate that MON 87751 contains one copy of T-DNA I containing the cry1A.105 and cry2Ab2 expression cassettes that is stably integrated at a single locus and is inherited according to Mendelian principles over multiple generations. No vector backbone sequences were detetcted
LMO characteristics
  • Food
Detection method(s)
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 11
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 4
Country's Decision or any other Communication Living modified organism(s) 9
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1
Living Modified Organism Recipient Organism” or “Parental Organisms 1