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Herbicide tolerant canola
BASF Plant Science GmbH
A canola (Brassica napus) event that is tolerant to the
imidazolinone herbicides imazamox and imazapyr. Canola event CLB-1
was developed using site-directed mutagenesis of the AHAS3 gene in
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
- Site Directed Mutagenesis
Acetohydroxy acid synthase 3 gene Promoter
Acetohydroxy acid synthase 3 gene
Acetohydroxy acid synthase 3 gene terminator
Canola event CLB-1 was developed through sequential site-directed
mutagenesis of the AHAS3 gene in canola cells. Mutated cells were
selected on the basis of tolerance to imazapyr and regenerated to
produce plants. Canola event CLB-1 was identified as a successful
mutant based on molecular analyses, herbicide efficacy and
agronomic evaluations and was thus chosen for further
This gene encodes an acetohydroxyacid synthase (AHAS) enzyme, which
is also referred to as an acetolactate synthase (ALS) enzyme. The
herbicide tolerance trait in canola event CLB-1 is conferred by two
point mutations, an alanine to threonine substitution at position
122 and a serine to asparagine substitution at position 653, in the
These mutations result in a modified AHAS3 enzyme carrying two
amino acid substitutions which render the enzyme insensitive to
imidazolinone herbicides, including imazamox and imazapyr.
The canola event CLB-1 imidazolinone herbicide tolerance trait is
under the control of the native AHAS3 promoter and is believed to
be constitutively expressed.