Potato modified for increased oxygen supply to tubers | BCH-LMO-SCBD-110680 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 13 Jul 2016
Living Modified Organism identity
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Potato modified for increased oxygen supply to tubers
B33-LegHg-3‘OCS 13; B33-LegHg-3‘OCS 45; B33-LegHg-3‘OCS 54 and B33-LegHg-3‘OCS 57
  • - Organization: Max Planck Institute of Molecular Plant Physiology (MPIMP) | BCH-CON-DE-49374-2
    Max Planck Institute of Molecular Plant Physiology (MPIMP)
    , Max-Planck-Institut für Molekulare Pflanzenphysiologie
    Am Mühlenberg 1
    14476 , Germany
    Phone: +49 331 567 - 80 ,
    Fax: +49 331 567 - 84 08,
The potato plants were genetically modified to express the Leghemoglobin gene from [i[Lotus japonicus[/i] under the control of a tuber-specific promoter.

The genetic modification is expected to lead to increased oxygen content in the tuber at up to a depth of 2 mm below the skin, leading to enlarged lenticels and an increased tuber starch content.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/breeding line: Desirée
Characteristics of the modification process
Derivative of pART27 (B33-LegHg-3'OCS)
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The papatin-B33 promoter from Solanum tuberosum mediates a tissue-specific expression of the leghaemoglobin gene in the tubers. The ocs terminator from Agrobacterium tumefaciens is used as a termination signal.

The nptII gene from Escherichia coli is expressed under the control of the promoter and terminator signal of the nopaline synthase gene from Agrobacterium tumefaciens and it was used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
  • Research
Detection method(s)
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