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Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein
PLANTA Angewandte Pflanzengenetik und Biotechnologie GmbH
The genetically modified sugarbeets was modified to constitutively
express the coat protein gene of the Beet Necrotic Yellow Vein
Virus (BNYVV), an important domain for encapsidation, vector
transmissibility and cell-to-cell transmission.
The constitutively expressed capsid protein gene gives rise to a
concentration of coat protein in every cell of the genetically
modified plant tissue which is far below the concentration found in
plants infected with BNYVV.
As a result of the genetic modification a BNYVV resistance of the
modified plants is expected.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
Resistance to antibiotics - Kanamycin
Resistance to diseases and pests - Viruses - Beet necrotic yellow virus (BNYV)
Selectable marker genes and reporter genes
- Agrobacterium-mediated DNA transfer
Nopaline Synthase Gene Promoter
Neomycin Phosphotransferase II
Nopaline Synthase Gene Terminator
The cDNA of the coat protein gene of the Beet Necrotic Yellow Vein
Virus (BNYVV) is expressed under the control of the double 35S
promoter of the Cauliflower mosaic virus (CaMV). The CaMV
35S-terminator is used as a termination signal.
The neomycin phosphotransferase gene (nptII), expressed under the
control of the promoter and termination signal of the nopaline
synthase gene (nos) of Agrobacterium tumefaciens, is used as a
The introduced nucleic acid is integrated in the genome of the
recipient organism at more than 8 insertion sites.
- Resistance to antibiotics
- Resistance to diseases and pests
- Beet necrotic yellow virus (BNYV)