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Living Modified Organism
(LMO)
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Soy modified for the expression of heat-labile enterotoxin, B subunit
EN
157
No
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Prague,Person:Dr. Oldrich Navratilsenior scientist,
, Czech RepublicPhone: + 420 225 106 455,Fax:Email: ueb@ueb.cas.cz,Website: http://www.ueb.cas.cz/en,Related OrganizationInstitute of Experimental Botany, AS CR (IEB)Academic or research institutePrague,
, Czech RepublicPhone: + 420 225 106 455,Fax:Email: ueb@ueb.cas.cz,Website: http://www.ueb.cas.cz/en,
Soy line No. 157 was modified to express a model immunogen for production in soybean seed with the insertion of the Escherichia coli LTB gene, which codes for the non-toxic beta subunit of the temperature labile enterotoxin. The gene under the control of the soybean seed-specific glycinin promoter and terminator.
In addition, a DNA fragment coding for hygromycin phosphotransferase was also inserted into the modified plant, which confers resistance to the antibiotic hygromycin, which is used for selection of transformed soy plants.
EN
In addition, a DNA fragment coding for hygromycin phosphotransferase was also inserted into the modified plant, which confers resistance to the antibiotic hygromycin, which is used for selection of transformed soy plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-10453-6 Organism Glycine max (Soybean, Soya bean, Soya, SOYBN)Crops
Variety: ‘Jack’
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pGly:ER-LTB
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- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-111061-1 Glycinin gene promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)Promoter
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BCH-GENE-SCBD-111062-1 Heat-labile enterotoxin, B subunit gene | Escherichia coli (ECOLX)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
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BCH-GENE-SCBD-111060-1 Glycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminator
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BCH-GENE-SCBD-111059-2 Ubiquitin-ribosomal protein gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-111058-1 Ubiquitin-ribosomal protein gene terminator | Solanum tuberosum (Potato, SOLTU)Terminator
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-108911-1 FLAG TagTransit signal
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BCH-GENE-SCBD-103023-2 KDEL ER retention signalTransit signal
The LTB gene was plant codon optimized and modified with the substitution of the bacterial signal peptide with a 20 aa signal peptide from A. thaliana basic chitinase. Furthermore a 14 aa extension comprising the FLAG epitope and KDEL ER retention signal, and flanking Bsp120 restriction sites were introduced by PCR.
The final sequence encoded a 137 aa protein of 15.5 kDa that yielded a 13.3 kDa LTB-FLAG protein after signal peptide cleavage.
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The final sequence encoded a 137 aa protein of 15.5 kDa that yielded a 13.3 kDa LTB-FLAG protein after signal peptide cleavage.
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- Research
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