Potato modified with an ethanol-inducible GUS reporter system | BCH-LMO-SCBD-111454 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 08 Nov 2016
Living Modified Organism identity
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Potato modified with an ethanol-inducible GUS reporter system
Alc-GUS-22, Alc-GUS-37, Alc-GUS-45
The LM potato was modified to express an ethanol inducible GUS gene from Escherichia coli.

This was carried out by introducing fragments of the ethanol regulon from Aspergillus nidulans into the potato genome in combination with the GUS gene which is under the control of a chimeric promoter consisting of a fragment of the 35S promoter of the Cauliflower Mosaic Virus and a fragment of the promoter of the alcohol dehydrogenase I gene, alcA, from A. nidulans

The fragment of the alcA promoter contains three binding sites for the transcription activator alcR from A. nidulans. which binds to the fragment of alcA of the chimeric promoter in the presence of ethanol thereby inducing transcription.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
Derivative of pBIN19
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The cDNA of the alcR transactivator gene is constitutively expressed under the control of the 35S promoter of the CaMV and the terminator sequence of the nopaline synthase gene from Agrobacterium tumefaciens.

The expression of the ß-glucuronidase gene is controlled by a chimeric promoter which consists of a 246 bp fragment of the A. nidulans alcA promoter, containing the three  binding sites for the transactivator AlcR, and a fragement of the CaMV-35S promoter (position –31 to +1). The CaMV 35S terminator signal is used for the termination of the transcription.

The neomycin phosphotransferase gene was expressed under the control of the promoter and terminator signals of the nopaline synthase gene from A. tumefaciens and is used as a selection marker.

The introduced nucleic acid is integrated into the genome of the recipient organism.
LMO characteristics
  • Research
Detection method(s)
Additional Information
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