MON-874Ø3-1 - Corn modified for increased ear biomass | BCH-LMO-SCBD-111522 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 19 Jan 2017 last updated: 18 Aug 2017
Living Modified Organism identity
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Corn modified for increased ear biomass
EN
MON87403
Yes
MON-874Ø3-1
Corn was modified with the insertion of the ATHB17 gene from Arabidopsis thaliana, which is a transcriptional repressor that has been shown to play an important role in the modulation of plant growth and development. As a result of the modification the resulting truncated form of the ATHB17 gene is unable to function as a transcriptional repressor since it lacks a functional repression domain therefore leading to increased ear biomass at an early reproductive phase compared to conventional control maize.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
PV-ZMAP5714
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The promoter region of this LMO consists a duplicated enhancer region from the cauliflower mosaic virus 35S promoter combined with the promoter of the act1 gene, that encodes Actin 1, from Oryza sativa.

In this modified organism, maize-specific splicing of the ATHB17 transcript results in a truncated protein, ATHB17Δ113, which is missing the first 113 N-terminal amino acids that are expressed in Arabidopsis thaliana. The ATHB17∆113 protein retains the ability to form homo- and hetero-dimers and bind to target DNA sequences like the full-length protein, however, it is unable to function as a transcriptional repressor because the protein lacks a functional repression domain.

The plasmid backbone also contained cp4 epsps and aadA expression cassettes. The cp4 epsps expression cassette is regulated by the act1 promoter from Oryza sativa, the act1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens. The aadA expression cassette is regulated by the bacterial promoter, and 3' untranslated region of an aminoglycosidemodifying enzyme, 3''(9)-O-nucleotidyltransferase from the transposon Tn7.

During transformation both the T-DNA and the cp4 epsps expression cassette were inserted into the maize genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate those plants that contain the ATHB17 expression cassette and not the cp4 epsps and aadA expression cassettes.

Based on sequencing, PCR and bioinformatic analysis, hte modified LMO contains a single copy of the ATHB17 expression cassette that is stably integrated at a single locus.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 3
Country's Decision or any other Communication Living modified organism(s) 4
Risk Assessment generated by a regulatory process Living modified organism(s) 4