Potato modified for increased tolerance to fungal pathogens | BCH-LMO-SCBD-111620 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 03 Feb 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for increased tolerance to fungal pathogens
EN
Dst9-3, Dst9-6, Dst22-6, Dst23-1, Dst23-15, Dst23-17 and Dst65-24
No
Potatoes were modified to express the ribonuclease barnase from Bacillus amyloliquefaciens under the control of the pathogen inducable prp-1 promoter from Solanum tuberosum. The genetically modified plants also constitutively express the barstar gene from Bacillus amyloliquefaciens which is a specific inhibitor protein for barnase.

As a result of the genetic modification the ribonuclease barnase is expressed upon pathogen infection, leading to the cell death of infected cells and restricting the spreading of the fungal pathogen. Since the prp1-1 promoter shows some activity even without pathogen infection, the constitutively expressed inhibitor barstar prevents the cell death of uninfected cells.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pTCV15 or pTCV17
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-111592-1 Pathogen-defence gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-111593-1 Alkaline phosphatase transit peptide | Escherichia coli (ECOLX)
    Transit signal
  • BCH-GENE-SCBD-14973-6 Barnase | Bacillus amyloliquefaciens (BACAM)
    Protein coding sequence | Changes in physiology and/or production (Reproduction, Male sterility)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-14974-7 Barstar | Bacillus amyloliquefaciens (BACAM)
    Protein coding sequence | Changes in physiology and/or production (Fertility restoration)
  • BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The pTCV17 vector was used to develop lines Dst9-3, Dst9-6 and Dst22-6 which contain a 237bp fragment of the prp1-1 promoter.

The pTCV15 vector was used to develop lines Dst23-1, Dst23-15, Dst23-17 and Dst65-24 which contain a 432bp fragment of the prp1-1 promoter.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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