Sinorhizobium meliloti modified for the expression of a reporter gene
Date of creation
2017-02-22 12:51 UTC (email@example.com)
Date of publication
2017-02-22 17:02 UTC (firstname.lastname@example.org)
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Sinorhizobium meliloti modified for the expression of a reporter
The genetically modified sinorhizobia constitutively express the
luciferase gene from Photinus pyralis coding for the
luciferin-4-monooxygenase which catalyses a light-producing
reaction and is therefore used as a reporter gene. Other than the
expression of the reporter gene, the modified S. meliloti
does not differ from the original strain.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
The S. meliloti strain Rm2011 was used for the transformation.
Sinorhizobium meliloti modified for the expression of a reporter gene and reduced growth
Selectable marker genes and reporter genes
The construct did not contain its own termination sequence. It was
site-specifically integrated downstream of the S. meliloti
recA Gene. The insertion site is located just before the endogenous
recA termination region, therefore, transcription termination would
be controlled by that sequence.
- Selectable marker genes and reporter genes
S. meliloti needs a host plant to grow and proliferate
properly. Here, the symbiotic interaction partner alfalfa,
Medicago sativa, is used as a host plant.