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Living Modified Organism (LMO)
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Sugarbeet modified for herbicide tolerance
EN
T120-7
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PersonPLANTAGrimsehlstraße 31Einbeck,
37574, GermanyPhone: +49 5561-3110,Fax: +49 5561-311243,Email: info@kws.de,Website: http://www.kws.de,Related OrganizationPLANTA Angewandte Pflanzengenetik und Biotechnologie GmbH ()Private sector (business and industry)Grimsehlstraße 31Einbeck,
37574, GermanyPhone: +49 5561-3110,Fax: +49 5561-311243,Email: info@kws.de,Website: http://www.kws.de,
Sugarbeets were modified to constitutively express a synthetic phosphinothricin acetyltransferase (pat) gene. As a result of the genetic modification the resulting sugarbeet is tolerant to L-phosphinothricin-containing herbicides.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12097-4 Organism Beta vulgaris (Common beet, Sugarbeet, BETMA)Crops
pOCA18/Ac
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoterPromoter
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase geneProtein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminatorTerminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene PromoterPromoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | (Bacteria)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-111871-2 COS site | Escherichia virus lambda (Lambda phage, LAMBD)Excision-integration site
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BCH-GENE-SCBD-111872-2 pMB1 origin of replication | (Bacteria)Plasmid Vector
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BCH-GENE-SCBD-111873-2 Tyrosine suppressor tRNA gene | (Bacteria)Protein coding sequence
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene TerminatorTerminator
The amino acid sequence of the pat gene is based on the pat gene from Streptomyces viridochromogenes but was codon optimized for expression in sugarbeet.
The following elements of the vector piAn7 were also transferred but are not expressed and are non-functional in sugarbeet:
*The cos-site of bacteriophage Lambda;
*The ori of pBR322; and
*The SupF t-RNA gene of E. coli.
The following elements of the vector piAn7 were also transferred but are not expressed and are non-functional in sugarbeet:
*The cos-site of bacteriophage Lambda;
*The ori of pBR322; and
*The SupF t-RNA gene of E. coli.
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