Record details

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Living Modified Organism identity

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LMO name

Sugarbeet modified for herbicide and fungus resistance

Transformation event

TAD13, TAD 18, TAD28, TAD33 and TAD44.

Developer(s)

Record #109234

Dieckmann Seeds

Dieckmann GmbH & Co. KG

Koverden 1
31737 Rinteln
Germany

Rinteln

Germany, 31737

Phone:	+49 5152 699 71-0
Fax:	+49 5152 699 71-29
Email:	info@dieckmann-seeds.de
Url:	Dieckmann Seeds

Description

Sugarbeets were modified to constitutively express phosphinothricin acetyltransferase (pat) gene. As a result of the genetic modification the resulting sugarbeet is tolerant to L-phosphinothricin-containing herbicides.

Furthermore, the plants were also modified to constitutively express the defensin-like protein 1 gene (AMP1) of Dahlia merckii. Defensins have antimicrobial properties, therefore the introduction of the AMP1 gene confers a resistance to pathogenic fungi to the plants.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12097

Beta vulgaris - Common beet, Sugarbeet, BETMA

Characteristics of the transformation process

Vector

pIG35SDM

Techniques used for the modification

Direct DNA transfer

Genetic elements construct

CaMV Enhanced 35S promoter

#100366

0.00 Kb

Phosphinothricin N-acetyltransferase gene

#14972

0.00 Kb

CaMV 35S terminator

#100290

0.00 Kb

CaMV Enhanced 35S promoter

#100366

0.00 Kb

Omega 5' untranslated leader

#104820

0.00 Kb

Defensin-like protein 1 gene

#111875

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The pIG35SDM plasmid was transferred into the plants as naked DNA via protoplast transformation. As a result, some of the transformation events have the entire transformation vector integrated into their genome while others only contain parts thereof.

In addition to the genetic elements listed above, the pIG35SDM plasmid also contained the following sequences which were also transferred into the modified sugarbeet but are non-functional in plants:

* The imidazoleglycerol phosphate dehydratase (his3) gene from Saccharomyces cerevisiae which is expressed as a selection marker in bacteria under the control of its native promoter and terminator sequences. Furthermore, due to the close proximity of these genes within the yeast genome, the transcription initiation site of the yeast ATP-dependent RNA helicase (ded1) gene and the first 6 bp of the ded1 coding sequence are included with of the 3' sequence of the his3 gene.

* Portions of the Lac operon from Escherichia coli, specifically: a 12 bp sequence of the N terminus of the lacZ gene, the promoter/operator region of the lacZ gene and 88 bp of the sequence of the C terminus of the lacI gene.

* The origin of replication of the pMB1 plasmid from E. coli.

Each of the transformation events contain up to three copies of the genes integrated into their respective genomes. There is no extrachromosomal replication of the genetic material.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Fungi
Resistance to herbicides
- Glufosinate

Common use(s)

Research