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Modified Organism
Roundup Ready Flex® Pima cotton
Record information and status
Record ID
111919
Status
Published
Date of creation
2017-05-19 06:42 UTC (gillian.colebatch@health.gov.au)
Date of last update
2017-05-19 23:20 UTC (gillian.colebatch@health.gov.au)
Date of publication
2017-05-22 16:27 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready Flex® Pima cotton
Transformation event
MON88913
Developer(s)
Regulatory Affairs
Monsanto Australia
PO Box 6051
St Kilda Road Central, VIC
Australia, 8008
Phone:+61 3 9522 7122
Email:melbourne.reception@monsanto.com
Url:Monsanto Australia
Description
Roundup Ready Flex® Pima cotton was produced via conventional breeding to transfer the genetic modification from Roundup Ready Flex® cotton (Gossypium hirsutum; MON88913) to unmodified G. barbadense, also known as pima cotton.

Roundup Ready Flex® Pima cotton therefore contains the same modification as MON88913, namely a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the cotton to survive otherwise lethal applications of glyphosate. The cp4 epsps gene introduced into MON 88913 and transferred by conventional breeding to Roundup Ready Flex® Pima cotton was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the CP4 EPSPS enzyme expressed by this gene is tolerant to glyphosate. 

As with the MON88913 parent, Roundup Ready Flex® Pima cotton contains two copies of the cp4 epsps gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
MON-88913-8 - Roundup Ready™ Flex™ cotton
Resistance to herbicides - Glyphosate
Show detection method(s)
Gossypium barbadense - Sea-island cotton, Egyptian cotton, GOSBA
Point of collection or acquisition of the recipient organism
G. barbadense cultivar Sipima 280 in Australia; G. barbadense cultivar W2490 in the United States
Characteristics of the transformation process
Vector
PV-GHGT35
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
FMV 35S Enhancer
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Elongation factor EF-1alpha promoter
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Elongation factor EF-1alpha Leader
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Elongation factor EF-1alpha Intron 1
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Chloroplast transit peptide 2
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5-enolpyruvylshikimate-3-phosphate synthase gene
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rbcS-E9 gene terminator
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CaMV 35S Enhancer
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Actin 8 Promoter
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Actin 8 Leader sequence
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Actin 8 Intron 1
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Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
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rbcS-E9 gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON88913 vector PV-GHGT35

Monsanto constructed the double border, binary plasmid vector PV-GHGT35 for the transformation of G. hirsutum cotton. The resulting modification was then transferred to G. barbadense cotton by conventional breeding.

The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two codon optimised cp4epsps gene cassettes: 1) the first cp4 epsps coding sequence (CS-cp4 epsps) under the regulation of a chimeric transcriptional promoter (P-FMV/Tsf1),Tsf1 leader and intron sequences (L-Tsf1 and I-Tsf1, respectively), a chloroplast transit peptide sequence (TS-ctp2) and a E9 transcript termination and polyadenylation sequence (T-E9) and 2) the second cp4 epsps coding sequence regulated by a chimeric transcriptional promoter(P-35S/act8), act8 leader and intron sequences (L-act8, and I-act8,respectively) and the same chloroplast targeting and transcript termination sequences as utilized in the first cp4 epsps gene expression cassette.

For additional information on this LMO, please refer to the records of the parental LMO.
LMO characteristics
Modified traits
Common use(s)
  • Fiber / Textile
Additional Information
Additional Information
The EPSPS enzyme is part of the shikimate pathway, an important biochemical pathway in plants involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed for growth and survival. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, since these organisms are unable to synthesize their own aromatic amino acids. Because the aromatic amino acid pathway is not present in mammals, birds, or aquatic life forms, glyphosate has little, if any, toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.