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Modified Organism
SYN-E3272-5 x MON-ØØØ21-9 - Modified thermostable alpha-amylase, herbicide-tolerant maize
Record information and status
Record ID
Date of creation
2017-06-07 13:06 UTC (dina.abdelhakim@cbd.int)
Date of last update
2018-02-20 18:26 UTC (dina.abdelhakim@cbd.int)
Date of publication
2018-02-20 18:26 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Modified thermostable alpha-amylase, herbicide-tolerant maize
Transformation event
3272 x GA21
Unique identifier
SYN-E3272-5 x MON-ØØØ21-9
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Url:Syngenta Seeds Germany
The stacked maize line SYN-E3272-5 and MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of amy797E alpha amylase, PMI, and EPSPS genes. The expression of these genes are expected to confer tolerance to glyphosate herbicide as well as the synthesis of thermostable alpha-amylase.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-E3272-5 - Enogen™ Maize
Mannose tolerance Selectable marker genes and reporter genes Thermostable alpha-amylase Use in industrial applications - Biofuel production
Show detection method(s)
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
pNOV7013 and pDPG434
Techniques used for the modification
  • Cross breeding
Genetic elements construct
27kD gamma-zein Promoter
0.68 Kb
amy797E alpha amylase
1.38 Kb
SEKDEL ER retention signal
0.01 Kb
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
CaMV 35S terminator
0.07 Kb
Ubiquitin gene promoter
1.99 Kb
Ubiquitin Intron 1
0.00 Kb
Phosphomannose Isomerase gene
1.18 Kb
Nopaline Synthase Gene Terminator
0.25 Kb
Rice actin 1 gene promoter
1.37 Kb
Rice actin 1, intron
0.00 Kb
Optimized Transit Peptide
0.37 Kb
5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
Nopaline Synthase Gene Terminator
0.24 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from 3272 vector pNOV7013
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.

DNA insert from GA21 vector pDPG434
The GA21 line of maize was modified to be tolerant of glyphosate-containing herbicides. The isolated endogenous maize epsps gene was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserted into the inbred AT maize variety.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Resistance to herbicides
  • Selectable marker genes and reporter genes
Common use(s)
  • Biofuel
Additional Information
Other relevant website address or attached documents

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