Insect resistant, herbicide tolerant maize

A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of 59122 x MIR604.  Insect resistance is conferred by the cry34Ab1, cry3A,  and cry35Ab1 genes from the common soil bacterium Bacillus thuringiensis, and glufosinate  herbicide tolerance through the pat gene. the event also contains the PMI gene which functions as a selection marker.

PHP17662 and pZM26

DNA insert from MIR604 vector pZM26
Cry3A gene was modified for enhanced expression in maize and such that the amino acid sequence of the synthetic version of Cry3A is the same as the native protein, except for the modified serine-protease recognition site. The pmi gene encodes the enzyme phosphomannose isomerase (PMI) that allows the plants to utilise mannose as a carbon source and is used as a selectable marker.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

For additional information on this LMO, please refer to the records of the parental LMOs.