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HCEM485 maize line was modified for glyphosate herbicide tolerance
by inserting a 6.0 kb maize genomic fragment containing a modified,
glyphosate tolerant form of the endogenous maize EPSPS encoding
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
EPSPS gene terminator
The maize EPSPS coding sequence was specifically modified by
site-directed mutagenesis to introduce two single-nucleotide
substitutions. These two point mutations resulted in a codon change
from threonine to isoleucine at position 102 (relative to the
amino acid sequence of the native maize EPSPS enzyme) and
a proline to serine change at position 106.
Molecular characterization of the insertion suggests that 4
complete copies of the fragment are inserted at a single location.