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Cassava modified for reduced starch metabolism
International Institute of Tropical Agriculture (IITA)
PMB 5320 Oyo Road 200001
Cassava was genetically modified for reduced starch metabolism in
the tuber after pruning, prior to crop harvest.
The modification was carried out through the insertion of an RNAi
construct that was designed to suppress endogenous expression of
each of alpha-amylase 3 which is involved in is involved in the
release of branched malto‐olicosaccharides from the starch granule.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
pCAMBIA1301_patatin AMY3 RNAi
- Agrobacterium-mediated DNA transfer
M27939 Intron Sequence
Nopaline Synthase Gene Terminator
Nopaline Synthase Gene Promoter
Hygromycin B phosphotransferase gene
Base pairs 57‐165 of the synthetic plant intron sequence M27939 are
integrated into the genetic construct.
The α-amylase sequence consist of the first 210 base pairs of the
- Changes in quality and/or metabolite content
- Resistance to antibiotics
- Selectable marker genes and reporter genes