Record details

Living Modified Organism identity

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LMO name

Insect resistant, herbicide tolerant maize

Transformation event

MON87427 x 59122

Unique identifier

MON-87427-7 x DAS-59122-7

Developer(s)

Record #14925

Monsanto

800 North Lindbergh Blvd.

St. Louis, MO

United States of America, 63167

Phone:	+ 1 314 694-1000
Fax:	+1 314 694-3080
Url:	Monsanto

Description

The stacked maize line MON87427 x 59122 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of EPSPS, PAT, Cry34Ab1 and Cry35Ab1 genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide and glyphosate herbicide.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #104758

MON-87427-7 - Maize modified for tissue selective glyphosate tolerance

Resistance to herbicides - Glyphosate

Show detection method(s)

Record #15165

DAS-59122-7 - Herculex™ RW Rootworm Protection maize

Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate

Show detection method(s)

Characteristics of the transformation process

Vector

PV-ZMAP1043 and PHP17662

Techniques used for the modification

Cross breeding

Genetic elements construct

Ubiquitin gene promoter

#100362

1.99 Kb

Cry34Ab1

#14994

0.37 Kb

Proteinase inhibitor II gene terminator

#100367

0.32 Kb

Peroxidase gene promoter

#100368

1.30 Kb

Cry35Ab1

#14995

1.15 Kb

Proteinase inhibitor II gene terminator

#100367

0.32 Kb

CaMV 35S promoter

#100287

0.55 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

CaMV 35S terminator

#100290

0.20 Kb

CaMV Enhanced 35S promoter

#100366

0.62 Kb

Hsp70 intron

#100359

0.80 Kb

Chloroplast transit peptide 2

#100365

0.23 Kb

5-enolpyruvylshikimate-3-phosphate synthase gene

#14979

1.37 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from MON87427, vector PV-ZMAP1043
MON87427 was modifies to express the CP4 EPSPS protein which confers tolerance to the herbicide glyphosate. The e35S-hsp70 promoter and intron combination is used to drive the tissue selective expression of the cp4 epsps gene resulting in CP4 EPSPS protein production in vegetative and female reproductive tissue, providing tolerance to glyphosate within these tissues.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Coleoptera (beetles)
  - Lepidoptera (butterflies and moths)
Resistance to herbicides
- Glufosinate
- Glyphosate
Selectable marker genes and reporter genes

Common use(s)

Food
Feed

Detection method(s)

External link(s)

MON-87427-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

DAS-59122-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

Additional Information

Other relevant website address or attached documents

MON-87427-7 x DAS-59122-7 - ISAAA