SPS-ØØØY9-7 - Innate® Hibernate Atlantic Potato | BCH-LMO-SCBD-112930 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 22 Dec 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Innate® Hibernate Atlantic Potato
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Y9
SPS-ØØØY9-7
  • - Person: SPS International | BCH-CON-NZ-111608-2
    Person
    SPS International
    5369 West Irving Street
    Boise, Idaho
    83706, United States of America
    Phone: +1 (208) 780-6066,
    Fax:
    Website:
    Related Organization
    ()
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Simplot Innate® Hibernate potato is was developed through the retransformation of the SPS-ØØØJ3-4 Innate™ Atlantic Potato line. The resulting organism silences genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes. It also expresses the late blight resistance gene which confers resistance to late blight disease.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-LMO-SCBD-109056-2 Living Modified Organism SPS-ØØØJ3-4 - Innate™ Atlantic Potato
    J.R. Simplot Company | Changes in quality and/or metabolite content (Pigmentation / Coloration, Protein and amino acids)
  • BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)
    Crops
Var. Atlantic
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Characteristics of the modification process
pSIM1678 and pSIM1278
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from vector pSIM1278
This DNA insert comprises two expression cassettes that were inserted into the pSIM1278 transformation vector.

The first cassette comprises fragments of both the asparagine synthetase-1 gene (Asn1) and the polyphenol oxidase-5 gene (Ppo5), arranged as inverted repeats between the Agp promoter of the ADP  glucose pyrophosphorylase gene (Agp) and the Gbss promoter of the granule-bound starch synthase gene (Gbss) and results in silencing of both the Ppo5 and Asn1 genes.

The second cassette is comprised of fragments of the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL), operably linked to the same Agp and Gbss promoters as the first cassette. The function of the second cassette is to silence the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL).

Molecular analyses indicated that the transformed organism contains contains 2 copies of the DNA insert, one of which is full and the other one lacking at least part of the R1/PhL promoter silencing cassette, and no vector backbone in the LMO..

Alignment of the integration site sequences to the MSU potato reference genome indicates the likely integration site is on chromosome 6 for pSIM1278 that did not disrupt any known genes. There are no ORFs covering the left junction associated with the pSIM1278 insert in Y9. A single ORF was identified spanning the left junction

DNA insert from vector pSIM1678
This DNA insert comprises two expression cassettes that were inserted into the pSIM1678 transformation vector.

The first cassette comprises of the Rpi-vnt1 expression cassette, which consists of the VNT1 protein coding region regulated by the native Rpi-vnt1 promoter and terminator sequences. VNT1 is an R-protein from the wild Solanum species, S. venturii, which functions in the recognition of P. infestans and elicits a hypersensitive response in potato, conferring resistance to late blight disease.

The second insert consists of a down-regulation cassette for the plant vacuolar invertase gene, VInv, consisting of sequence from the potato VInv gene arranged as an inverted repeat and flanked by opposing plant promoters, pGbss and pAgp. Vacuolar invertase converts sucrose into glucose and fructose. Its down regulation prevents excess darkening during frying and contributes to low acrylamide potential

Molecular analyses indicated that the transformed organism contains a single nearly complete copy of the DNA insert at a single locus flanked by an additional, partial VInv cassette and no vector backbone in the LMO.

Alignment of the integration site sequences to the MSU potato reference genome indicates the likely integration site is on chromosome 5 for pSIM1678. There are no ORFs covering the right junction associated with the pSIM1678 insert in Y9. A single ORF was identified spanning the left junction.

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None of the X17 junction ORFs were identified as homologs of known toxins or allergens. Each Y9 insert is shown to be stable during vegetative propagation.
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LMO characteristics
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  • Food
Detection method(s)
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Additional Information
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Risk Assessment generated by a regulatory process Living modified organism(s) 3
Country's Decision or any other Communication Living modified organism(s) 2