Recombinant glycolate dehydrogenase (fused subunits DEF) | Escherichia coli (ECOLX) | BCH-GENE-SCBD-114683 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

published: 10 May 2019 last updated: 19 Jun 2019
General information
Recombinant glycolate dehydrogenase (fused subunits DEF)
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  • DEFp
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GlyDH
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Protein coding sequence
No
Donor organism
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Characteristics of the protein coding sequence
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Glycolate dehydrogenase converts 2-phosphoglycolate to glycerate and CO2. In plant biotechnology applications, the enzyme can be used used to increase photosynthetic rates by reducing the the toxic effects of photorespiration (Ribulose-1,5-bisphosphate-caboxylase/-oxygenase (Rubisco) to binding O2 instead of CO2).

The recombinant glycolate dehydrogenase polyprotein (DEFp) by fusing the corresponding E. coli glcD (1493bps),glcE (1046 bps), and glcF (1220 bps) cDNAs with intervening flexible (Gly4Ser) 3 linkers. The fused polyprotein ensures the protein subunits are produced in stoichiometric amounts.
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