Glycolate dehydrogenase converts 2-phosphoglycolate to glycerate
and CO2. In plant biotechnology applications, the enzyme can be
used used to increase photosynthetic rates by reducing the the
toxic effects of photorespiration
(Ribulose-1,5-bisphosphate-caboxylase/-oxygenase (Rubisco) to
binding O2 instead of CO2).
The recombinant glycolate dehydrogenase polyprotein (DEFp) by
fusing the corresponding E. coli glcD (1493bps),glcE (1046 bps),
and glcF (1220 bps) cDNAs with intervening flexible (Gly4Ser) 3
linkers. The fused polyprotein ensures the protein subunits are
produced in stoichiometric amounts.