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Modified Organism
TUC-873RH-7 - Herbicide-tolerant sugar cane
Record information and status
Record ID
115057
Status
Published
Date of creation
2019-07-19 13:04 UTC (austein.mcloughlin@cbd.int)
Date of last update
2021-05-03 20:15 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-05-03 20:15 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant sugar cane
Transformation event
TUC-873RH-7
Unique identifier
TUC-873RH-7
Developer(s)
Estación Experimental Agroindustrial Obispo Colombres (EEAOC)
San Miguel de Tucumán, Tucumán
Argentina, T4101XAC
Phone:+54 381 452 1000
Fax:+54 381 452 1008
Url:Estación Experimental Agroindustrial Obispo Colombres (en Español)
Description
Sugarcane (Saccharum sp.) was modified to contain Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase for tolerance to glyphosate. An Escherichia coli neomycin phosphotransferase II cassette was also included for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Saccharum sp. - Sugar cane
Point of collection or acquisition of the recipient organism
Sugarcane cultivar RA 87-3
Characteristics of the transformation process
Vector
pBluescript II KS(+)
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Rice actin 1 gene promoter
0.00 Kb
 
 
Chloroplast Transit Peptide 4
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified sugarcane contains two gene cassettes: Escherichia coli (nptII) and Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate (cp4-epsps).

The nptII coding sequence is under control of a Zea mays ubiquitin promoter and an A. tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at elevated levels in all tissue types.

The cp4-epsps coding sequence is under control of an Oryza sativa actin 1 promoter and Cauliflower mosaic virus 35S terminator. To the 5' end of the cp4-epsps sequence is a Petunia hybrida chloroplast transit peptide 4 sequence to target the translated EPSPS protein to the chloroplast. The rice promoter is expected to facilitate high levels of transcription in all tissue types.

Note:
- Southern blot analysis confirmed that 9 insertions of the transgenes occurred.
- Whole genome sequencing, Sanger sequencing, and PCR analysis confirmed that the vector backbone was not present in the genome, and that no deletions nor rearrangements occurred.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Biofuel
Additional Information