The modified sugarcane contains two gene cassettes: Escherichia
coli (nptII) and Agrobacterium tumefaciens
The nptII coding sequence is under control of a Zea
mays ubiquitin promoter and an A. tumefaciens
nopaline synthase terminator. Due to the constitutive nature of the
promoter, transcription is expected to occur at elevated levels in
all tissue types.
The cp4-epsps coding sequence is under control of an
Oryza sativa actin 1 promoter and Cauliflower mosaic
virus 35S terminator. To the 5' end of the cp4-epsps
sequence is a Petunia hybrida chloroplast transit peptide
4 sequence to target the translated EPSPS protein to the
chloroplast. The rice promoter is expected to facilitate high
levels of transcription in all tissue types.
- Southern blot analysis confirmed that 9 insertions of the
- Whole genome sequencing, Sanger sequencing, and PCR analysis
confirmed that the vector backbone was not present in the genome,
and that no deletions nor rearrangements occurred.