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Glyphosate and Glufosinate tolerant soybean
Ocampo 210 bis
Predio CCT Rosario
(2000) Rosario, Santa Fe, Argentina
Tel: (54) 341 4861100
The soybean was modified to express Agrobacterium
tumefaciens CP4 EPSPS and Streptomyces
viridochromogenes PAT proteins for tolerance to glyphosate and
glufosinate herbicides, respectively. The bacterial CP4 EPSPS
functions similarly to the endogenous enzyme, but has a greatly
reduced affinity for glyphosate. The PAT protein detoxifes
L-phosphinothricin (glufosinate) via the acetylation of the primary
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
Elongation Factor 1 alpha promoter
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
rbcS-E9 gene terminator
Phosphinothricin N-acetyltransferase gene
Transcription of the Agrobacterium tumefaciens
5-enolpyruvylshikimate-3-phosphate synthase (cp4
epsps) commences from the Glycine max Elongation
factor 1 alpha promoter and terminates at the Pisum
sativum ribulose-1,5-bisphosphate carboxylase, small
subunit (rbcS E9) terminator. An Arabidopsis thaliana
chloroplast peptide 2 segment was added to the 5' end of cp4
epsps to target the final protein product to the chloroplast.
The soy EF1α promoter is expected to promote high levels of
Streptomyces viridochromogenes Phosphinothricin
N-acetyltransferase (pat) gene is under control of the
Cauliflower Mosaic Virus (CaMV) 35S promoter and terminator.
Elevated levels of transcription of pat are expected due
to the CaMV 35S promoter. The protein is expected to remain in the
cytoplasm of the plant cells.