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Living Modified Organism (LMO)
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Glyphosate and Glufosinate tolerant soybean
EN
DBN9004
Yes
DBN-Ø9ØØ4-6
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Organization:Bioceres S.A. ()Ocampo 210 bis Predio CCT Rosario (2000) Rosario, Santa Fe, Argentina Tel: (54) 341 4861100Rosario,
, ArgentinaPhone: + 54 341 4861100,Fax:Email:Website: http://www.bioceres.com.ar/eng/,
The soybean (Glycine max) was modified for herbicide tolerance. For tolerance to glyphosate, the soybean expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which has a reduced binding affinity for the herbicide and allows the continued functioning of the shikimate pathway. For tolerance to glufosinate (L-phosphinothricin), the soybean expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase gene, which detoxifies the herbicide through acetylation of the primary amino group.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-10453-6 Organism Glycine max (Soybean, Soya bean, Soya, SOYBN)Crops
EN
pDBN4003
EN
- Agrobacterium-mediated DNA transfer
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1.012 kb
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0.228 kb
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1.368 kb
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0.643 kb
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0.530 kb
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0.552 kb
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0.195 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-115065-1 Elongation Factor 1 alpha promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)Promoter
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The modified soybean contains two gene cassettes: Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).
Transcription of the epsps commences from a Glycine max elongation factor 1 alpha promoter and terminates at a Pisum sativum ribulose-1,5-bisphosphate carboxylase small subunit terminator. An Arabidopsis thaliana chloroplast transit peptide 2 sequence was added to the 5' end of epsps coding sequence to target the translated protein to the chloroplast. Due to the nature of the promoter, high levels of transcription are expected.
Transcription of the pat coding sequence under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a CaMV terminator. Elevated levels of transcription of pat are expected due to the CaMV 35S promoter. The protein is expected to remain in the cytoplasm of the plant cells.
Transcription of the epsps commences from a Glycine max elongation factor 1 alpha promoter and terminates at a Pisum sativum ribulose-1,5-bisphosphate carboxylase small subunit terminator. An Arabidopsis thaliana chloroplast transit peptide 2 sequence was added to the 5' end of epsps coding sequence to target the translated protein to the chloroplast. Due to the nature of the promoter, high levels of transcription are expected.
Transcription of the pat coding sequence under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a CaMV terminator. Elevated levels of transcription of pat are expected due to the CaMV 35S promoter. The protein is expected to remain in the cytoplasm of the plant cells.
EN
- Biofuel
- Feed
- Food
EN
EN
- Euginius: DBN9004 event [ English ]
- (Chinese | 中文) Patent - WO2017215328A1 (PDF) [ English ]
- FAO GM Foods Platform: DBN-Ø9ØØ4-6 [ English ]
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