| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Information Resource
Record information and status
Record ID
115389
Status
Published
Date of creation
2020-02-13 19:23 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-02-13 19:23 UTC (austein.mcloughlin@cbd.int)

General Information
Title
Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR)
Author
Toshitsugu Fujita, Daisuke Motooka, Hodaka Fujii
Language(s)
  • English
Publication date
2019-06-21
Subject
Summary, abstract or table of contents
Abstract
Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) is amethod that suppresses PCR amplification of target DNA in an ORN-specific manner. In this study, we examined whether ORNi-PCR can be used to enrich desirable DNA sequences from a DNA mixture by suppressing undesirable DNA amplification. ORNi-PCR enriched edited DNA sequences from a mixture of genomic DNA subjected to genome editing. ORNi-PCR enabled more efficient analysis of the types of insertion/deletion mutations introduced by genome editing. In addition, ORNi-PCR reduced the detection of 16S ribosomal RNA (16S rRNA) genes in 16S rRNA gene-based microbiome profiling, which might permit a more detailed assessment of populations of other 16S rRNA genes. Enrichment of desirable DNA sequences by ORNi-PCR may be useful in molecular biology, medical diagnosis, and other fields.

Methods manuscript
Thematic areas
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
doi: 10.1093/biomethods/bpz009
Publisher and its location
Biology Methods & Protocols
Oxford University Press
Rights
Open access
Format
PDF - 6 pages (368 KB)
Source
Original document
Keywords and any other relevant information
PCR; ORNi-PCR; oligoribonucleotide; genome editing; CRISPR; detection; enrichment