SPS-ØØØZ6-5 - Elevate potato | BCH-LMO-SCBD-115591 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 29 May 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Elevate potato
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Z6
SPS-ØØØZ6-5
  • - Organization: J.R. Simplot Company () | BCH-CON-SCBD-106427-1
    Organization
    J.R. Simplot Company ()
    Private sector (business and industry)
    5369 West Irving Street
    Boise, ID
    83706, United States of America
    Phone: +1 (208) 780-6066,
    Fax:
    Email:
    Website:
The Elevate potato (Event Z6) was created through the Agrobacterium-mediates transformation of the Invigorate potato (Event V11). The modified potato was modified for Phytophthora infestans resistance, reduced enzymatic blackening and low acrylamide potential. To achieve fungal resistance, the modified potato expresses a resistance gene from a wild potato, Solanum venturii (Rpi-vnt1). To reduce blackening of the potato tissue, an RNA interference (RNAi) cassette is expressed that targets the endogenous polyphenol oxidase-5 gene. To reduce the acrylamide potential, decrease levels of reducing sugars and asparagine were achieved through RNAi silencing of vacuolar invertase, water dikinase R1 and asparagine synthetase-1.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)
    Crops
  • BCH-LMO-SCBD-115075-1 Living Modified Organism SPS-ØØV11-6 - Innate® Invigorate Snowden
    J.R. Simplot Company | Changes in quality and/or metabolite content (Pigmentation / Coloration, Protein and amino acids)
Z6 was produced through the transformation of V11 with vector pSIM1678.
V11 was produced through the transformation of potato variety Snowden with vector pSIM1278.
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Characteristics of the modification process
pSIM1278 and pSIM1678
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from pSIM1278 (V11 genome)
The T-DNA insert contains two RNA interference (RNAi) cassettes for the potato genes: asparagine synthase-1 (Asn1) - polyphenol oxidase 5 (Ppo5) and phosphorylase-L (PhL) - Alpha-glucan water dikinase R1 (R1).

Asn1-Ppo5:
Transcription commences from both promoters, the potato ADP glucose pyrophosphorylase promoter (P-Agp) and the potato granule bound starch synthase promoter (P-Gbssp), which are in a convergent orientation relative to each other. The transcripts produced have inverted repeats of Asn1 and Ppo5 segments, separated by a spacer sequence derived from the potato genome. After transcription, the transcripts form double stranded RNA (dsRNA) molecules due to the homology of the sequences. The RNA molecules are then sufficient to trigger an RNAi response.

pPhL-R1:
Transcription is also directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the promoter segments of PhL and R1, as well as a spacer derived from the potato genome. Post-transcription, the RNA molecules can form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- The promoters are tuber-specific and expected to be most active in the tuber tissue.
- The transcripts are not expected to be translated into proteins.
- No specific terminators were added to the cassettes and thus the transcripts are not expected to have poly(A) tails.
- No marker genes (e.g. kanamycin or hygromycin resistance) are present.
- Southern blotting confirmed that the V11 genome contains a single T-DNA insertion.
- No vector backbone was incorporated into the V11 genome.

DNA insert from pSIM1678
The T-DNA insert contains two gene cassettes: Phytophora infestans resistance genes (Rpi-vnt1) from Solanum venturii and endogenous vacuolar invertase (vInv) RNAi.

Rpi-vnt1
Transcription of Rpi-vnt1 is under control of the native promoter and terminator.

vInv
Transcription is directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the vInv, as well as a spacer, also derived from vInv. Post-transcription, the RNA molecules form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- Southern blot analysis indicated that a single full length insertion of the T-DNA was inserted into the Z6 genome
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LMO characteristics
Post-transcription, the sequence homology (inverted repeats) base pair to from hairpin RNA (hpRNA). The double strandedness of the hpRNA triggers an RNAi response. First, DICER recognizes the double stranded RNA of the hpRNA, associates with the hpRNA and fragments the hpRNA into ~21-23 basepair segments, termed small interfering RNAs (siRNA). ARGONAUTE family proteins bind the siRNA, unwind one of the strands to activate the RISC complex, which uses the remaining siRNA strand to target transcripts with sequence homology for degradation and thus results in gene silencing. Thus, the modified potato silences the gene expression of the following endogenous genes: Asn1, Ppo5, PhL, R1 and vInv.
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  • Food
Detection method(s)
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Additional Information
Potatoes have the potential to contain acrylamide, a toxic/carcinogenic compound formed during exposure to high heat (i.e. during cooking). Acrylamide is formed through the Maillard reaction (non-enzymatic browning), where the carbonyl group of the  reducing sugar reacts with the nucleophilic amino group of the amino acid.
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