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Modified Organism
Cassava brown streak disease resistant cassava
Record information and status
Record ID
115624
Status
Published
Date of creation
2020-06-26 14:06 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-06-26 14:06 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cassava brown streak disease resistant cassava
Transformation event
pCRNAi-CBSV-CP
Developer(s)
Prof. Hassan Karakacha Were
Dean, School of Agriculture, Veterinary Science and Technology
Masinde Muliro University of Science and Technology (MMUST)
Kakamega
Kenya
Phone:(+254) (724) (972207)
Email:hwere@mmust.ac.ke
Description
The cassava was modified for resistance to Cassava brown streak disease (CBSD) by introducing an RNA interference cassette that targets Cassava brown streak virus (CBSV) coat protein (CP). The production of hairpin RNA by the host cells trigger an RNAi response that is expected to target viral transcripts and prevent viral assembly and thus further infection. Due to conservation of the CP sequence between CBSV and Ugandan cassava brown streak virus, the modified cassava is expected to be resistant to the causal agents of CBSD. A selectable marker, Escherichia coli hygromycin B phosphotransferase, was additionally included for hygromycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Related LMOs
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pCAMBIA1300
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Coat protein
0.53 Kb
 
 
M27939 Intron Sequence
0.20 Kb
 
 
Coat protein
0.53 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA interference cassette
The modified cassava contains an RNA interference (RNAi) cassette designed to target Cassava brown streak virus coat protein (CP). Transcription is initiated from the Cauliflower mosaic virus (CaMV) 35S promoter and terminates at the CaMV 35S terminator. The transcript contains two segments (sense and antisense) of CP separated by a plant synthetic intron. Post-transcription, the intron functions as a loop and allows the sense and antisense segments of CP to base pair, forming hairpin RNA (hpRNA). The hpRNA acts as double stranded RNA (dsRNA), which triggers an RNAi response and the host cell machinery will target CP viral transcripts for degradation.

Note:
- The segment of CP corresponds to positions 538 to 1063 of the CBSV (GenBank accession JN091565.1).
- The source of the CP sequence is CBSV strain TAZ-DES-01.
- Due to the RNAi response, no protein translation is expected from the RNAi cassette.

Selectable marker
Transcription of Escherichia coli hygromycin B phosphotransferase is under transcriptional control of the CaMV 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Resistance to Cassava brown streak virus
Common use(s)
  • Food
Additional Information
Additional Information
RNA interference
An RNAi response is an anti-viral response triggered by the recognition of dsRNA. Host DICER recognizes dsRNA, cleaving the dsRNA into small interfering RNA (siRNA), roughly 21-23 bp long (size is host dependent). The siRNA is then bound by ARGONAUTE family proteins, which unwind the duplex, leaving a single strand of the siRNA, and activating the RISC complex. The RISC complex targets transcripts with homology to the siRNA and degrades them.

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism4 records