The Sphingobium herbicidovorans
R-2,4-dichlorophenoxypropionate dioxygenase was engineered to have
increased thermostability and enzymatic activity. The enzyme
inactivates quizalofop (active against monocots) and
2,4-dichlorophenoxyacetic acid herbicides using α-ketoglutarate and
molecular oxygen to produce the inactivated herbicide, succinate
and carbon dioxide. The enzyme may also act on other
aryloxyphenoxypropionate and synthetic auxin herbicides.