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Maharashtra Hybrid Seeds Company Limited (Mahyco)
Jalna - Aurangabad Road,
Post Box no. 76,
The okra (Abelmoschus esculentus) was modified for
resistance to Lepidoptera pests (e.g. spotted bollworm) through the
expression of Bacillus thuringiensis Cry1Ac. The protein
forms pores in the midgut lining of susceptible pests, leading to
cell lysis and septicemia. Additionally, the okra contains an
Escherichia coli neomycin phosphotransferase II cassette
for kanamycin selection during transformation.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
CaMV Enhanced 35S promoter
Neomycin Phosphotransferase II
The modified okra includes two gene cassettes: Bacillus
thuringiensis cry1Ac and Escherichia coli neomycin
phosphotransferase II (nptII).
The cry1Ac is under control of the Cauliflower mosaic
virus (CaMV) 35S enhanced promoter and a poly(A) terminator.
Due to the constitutive nature of the promoter, transcription is
expected to occur at high levels.
Transcription of nptII is under control of the CaMV 35S
promoter and 35S terminator. Again, transcription is expected to
occur at high levels due to the nature of the viral promoter.
- Resistance to antibiotics
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
- Selectable marker genes and reporter genes