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Living Modified Organism (LMO)
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Dhara Mustard Hybrid-11
EN
DMH-11 (Varuna bn 3.6 × EH-2 modbs 2.99)
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New Delhi,OrganizationUniversity of Delhi ()Academic or research institute, Centre for Genetic Manipulation of Crop Plants (CGMCP)
, IndiaPhone:Fax:Email:
The hybrid DMH-11 Indian mustard (Brassica juncea) was produced through crossing two modified parental lines for restored male-fertility and herbicide tolerance. The mustard expresses expresses both Bacillus amyloliquefaciens barnase, an RNase, and barstar, an inhibitor of barnase, in the tapetum cell layer of the pollen sac during anther development. Without barstar, the non-specific nature of barnase would degrade the RNA and prevent the pollen from developing. The expression of barnase allowed the developers to control crosses of the parental plants. In this specific cross, fertility (pollen production) was restored through the introduction of barstar. Overall, the resulting heterosis is expected to improve productivity of the hybrid mustard.
For glufosinate tolerance, the mustard expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation.
For glufosinate tolerance, the mustard expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115905-1 Organism Brassica juncea - Indian mustard, Brown mustard, Chinese mustard, Leaf mustard, Vegetable mustard, Mustard greens, BRAJU
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BCH-LMO-SCBD-115909-2 Living Modified Organism Male sterile Indian mustardChanges in physiology and/or production - Reproduction - Male sterility Resistance to herbicides - Glufosinate, Imidazolinone, Sulfonylurea
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BCH-LMO-SCBD-115910-1 Living Modified Organism Fertility restorer Indian mustardChanges in physiology and/or production - Fertility restoration Resistance to herbicides - Glufosinate
Kindly refer to parental records for more information.
pPZP200
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14973-6 BarnaseProtein coding sequence | Changes in physiology and/or production (Reproduction, Male sterility)
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BCH-GENE-SCBD-14974-7 BarstarProtein coding sequence | Changes in physiology and/or production (Fertility restoration)
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase geneProtein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-115906-1 Topoisomerase - Pisum sativum - Garden pea, PEA
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BCH-GENE-SCBD-101407-6 pTA29 pollen specific promoter | (Tobacco plant)Promoter
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BCH-GENE-SCBD-100287-7 CaMV 35S promoterPromoter
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BCH-GENE-SCBD-103886-2 5' Untranslated Leader of AMV RNA4 | (Alfalfa mosaic virus, AMV)Leader
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoterPromoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminatorTerminator
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene TerminatorTerminator
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BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | (Thale cress)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
Insertion related to Varuna bn 3.6
The parental genome contains two gene cassettes: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) and Bacillus amyloliquefaciens barnase.
The bar coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter with an Alflafa mosaic virus (AMV) leader and an Rhizobium radiobacter octopine synthase gene terminator. The AMV leader sequence enhances expression of bar.
Barnase is under control of a Nicotiana tabacum TA29 promoter and a CaMV 35S terminator. The TA29 promoter is active only in the tapetum cell layer of the pollen sac during anther development (male-specific expression).
A spacer fragment can be found between the two gene cassettes to prevent 'leaky' expression of barnase from the CaMV promoter. It is comprised of Pisum sativum topoisomerase (3kb) and Arabidopsis thaliana acetohydroxy acid synthase (2 kb) fragments. Each fragment contains truncations on the 3' and 5' ends. No open reading frames were created during the fusion of the two fragments and thus are not expected to encode a functional product. Note: The arrangement of the insert is unclear.
Note:
- Southern blot and segregation analyses indicated that the genome contains a single insertion.
- Sequencing analysis indicated that the T-DNA integrated was identical to the sequences in the vector.
- The T-DNA was found to be integrated in A9 Linkage Group on the 'A' genome between Bra32488 and Bra32489 genes.
Insertion related to EH-2 modbs 2.99
The parental genome contains two gene cassettes: (bar) and B. amyloliquefaciens barstar.
The bar coding sequence is under control of a CaMV enhanced 35S promoter and R. radiobacter octopine synthase terminator.
Barstar is under control of a N. tabacum TA29 pollen specific promoter and a CaMV 35S terminator. The TA29 promoter is active only in the tapetum cell layer of the pollen sac during anther development (male-specific expression). The coding sequence was codon optimized for expression in plants.
Note:
- Southern blot and segregation analyses indicated that the genome contains a single insertion
- DNA sequence analysis indicated that the insertion occurred in the 'B' genome.
For more information, kindly refer to the parental records.
The parental genome contains two gene cassettes: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) and Bacillus amyloliquefaciens barnase.
The bar coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter with an Alflafa mosaic virus (AMV) leader and an Rhizobium radiobacter octopine synthase gene terminator. The AMV leader sequence enhances expression of bar.
Barnase is under control of a Nicotiana tabacum TA29 promoter and a CaMV 35S terminator. The TA29 promoter is active only in the tapetum cell layer of the pollen sac during anther development (male-specific expression).
A spacer fragment can be found between the two gene cassettes to prevent 'leaky' expression of barnase from the CaMV promoter. It is comprised of Pisum sativum topoisomerase (3kb) and Arabidopsis thaliana acetohydroxy acid synthase (2 kb) fragments. Each fragment contains truncations on the 3' and 5' ends. No open reading frames were created during the fusion of the two fragments and thus are not expected to encode a functional product. Note: The arrangement of the insert is unclear.
Note:
- Southern blot and segregation analyses indicated that the genome contains a single insertion.
- Sequencing analysis indicated that the T-DNA integrated was identical to the sequences in the vector.
- The T-DNA was found to be integrated in A9 Linkage Group on the 'A' genome between Bra32488 and Bra32489 genes.
Insertion related to EH-2 modbs 2.99
The parental genome contains two gene cassettes: (bar) and B. amyloliquefaciens barstar.
The bar coding sequence is under control of a CaMV enhanced 35S promoter and R. radiobacter octopine synthase terminator.
Barstar is under control of a N. tabacum TA29 pollen specific promoter and a CaMV 35S terminator. The TA29 promoter is active only in the tapetum cell layer of the pollen sac during anther development (male-specific expression). The coding sequence was codon optimized for expression in plants.
Note:
- Southern blot and segregation analyses indicated that the genome contains a single insertion
- DNA sequence analysis indicated that the insertion occurred in the 'B' genome.
For more information, kindly refer to the parental records.
- Food
- Safety-assessment-report-on-GE-Mustard_0.pdf [ English ]
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