Bt cauliflower | BCH-LMO-SCBD-115912 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 23 Feb 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Bt cauliflower
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CFE-4
  • - Organization: Maharashtra Hybrid Seeds Company Limited (Mahyco) | BCH-CON-SCBD-115887-1
    Organization
    Maharashtra Hybrid Seeds Company Limited (Mahyco)
    Private sector (business and industry)
    , Head Office
    Jalna - Aurangabad Road, Post Box no. 76, Dawalwadi, Jalna
    Dawalwadi, Maharashtra
    431203, India
    Phone: +91 02482-262471,
    Fax: +91 02482-26002,
    Email: info@mahyco.com,
The cauliflower (Brassica oleracea) was modified for resistance to Lepidoptera pests through the introduction and expression of Bacillus thuringiensis crystal protein, Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the cauliflower contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-115913-1 Organism Brassica oleracea var. botrytis - Cauliflower, Broccoflower, Calabrese, Romanesci broccoli
Brassica oleracea Botrytis group
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Characteristics of the modification process
pC2300ve10518
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  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified cauliflower contains two gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII) and Bacillus thuringiensis cry1Ac.

Transcription of nptII commences from a Cauliflower mosaic virus (CaMV) 35S promoter and terminates at a CaMV 35S terminator. Due to the viral promoters, transcription of nptII is expected to occur at elevated levels in all plant tissues.

Transcription of cry1Ac is controlled by a CaMV enhanced 35S promoter. Transcription is expected to occur at very high levels in all tissues due to the enhanced nature (duplicated enhancer) of the viral promoter.
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LMO characteristics
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  • Food
Detection method(s)
See provision patent attached in the 'Additional Information' section below.
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Additional Information
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