The genetically modified organism above mentioned shall be used only in the way described in the application submitted to the Ministry of the Environment (hereinafter “MoE”) on October 5, 2006, and supplemented with a submission delivered to MoE on December 21, 2006, and only when keeping all given conditions especially as follows:
- Every handling with the genetically modified material shall be under conditions minimising a possibility of transgene escape into the environment.
- To minimise possible spread of the transgene through seeds all the fruits shall be harvested even before getting mature. Nevertheless, monitoring of the possible transgene escape into the environment shall be carried out by testing wild plants of plums, sloes and myrobalans.
- In laboratory analyses of plant samples (leaves, bark) – ELISA, RT-PCR tests – it must be taken into account that the taken samples have no ability to propagate or transmit the genetic material, and therefore every handling with them is not considered as the use of GMOs under
§ 3 par. 2 of the Act. However, fruits must be handled in a way preventing the possible spread of the transgene, for example a thermal inactivation thereof. Surplus parts of samples from an analysis shall be autoclaved, which is a standard process of handling with quarantine organisms in the Virology Departments of the Crop Research Institute despite a minimal possibility of spreading these organisms. Other plant remains that could be under certain conditions capable to propagate and transmit a heritable material, it means lignified sprouts that can be used as scions, shall be removed by their burning down directly at the field trial site, and the ash shall be put in the soil on the spot.

- The assessment of the field trial shall be carried out every year during a vegetation season from April to August. The assessment will proceed with the use of DAS-ELISA and RT-PCR methods. Laboratory tests shall be performed with three leaf samples.
- The plant material shall be transported in the contained space of a transportation vehicle under the supervision of a responsible person. All staff getting into touch with GM plums shall be trained by a professional consultant every year on rules for the use of GMOs. The samples could be transferred from the site of the field trial only for purpose of a laboratory analysis (see above). Laboratory analysis of taken GM plum samples shall be performed in laboratories of the Crop Research Institute (CRI), a phytotron, ground-floor, door No. 004B 005.
- According to the opinion of the Bee Research Institute it is necessary to keep the distance 1000 m between the transgenic plum trees and other plum trees in order to prevent the spread of transgene into the environment, which has been ensured. Moreover, at the flowering time no bee colonies can occur 700 m apart from transgenic plants, which has been controlled and met.
- After the field trial is finished, the transgenic trees shall be torn out and incinerated on the spot. The plot shall be deeply ploughed and monitored for possible occurrence of young shoots or plum saplings following two years. These pertinent young shoots shall be destroyed chemically with the total herbicide or mechanically.

- The authorised person must whenever during the use of GMOs provide with samples of given genetically modified organism or its genetic material on request of the Ministry of the Environment or a laboratory according to § 28 par. 1 f) of the Act.