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Record information and status
Record ID
Date of creation
2021-03-23 14:32 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-03-23 14:33 UTC (austein.mcloughlin@cbd.int)

General Information
Qualitative PCR-based detection of genetically modified soy and maize products in Iran
Payam Safaei, Sassan Rezaie, Mahmood Alimohammadi, Setareh Agha Kuchak Afshari, Mehrangiz Mehdizadeh, Ebrahim Molaee Aghaee
Author’s contact information
Ebrahim Molaee Aghaee
Department of Environmental Health, Food Safety Division
Faculty of Public Health, Tehran University of Medical Science
Tehran, Iran

Kindly refer to the attached publication for contact's email address
  • English
Publication date
Summary, abstract or table of contents
Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to provide consumers with information. For this purpose, an accurate qualitative polymerase chain reaction (PCR) method was applied to investigate GM food products from Iran. In present study, a total of 90 non-labeled soy and maize samples were collected from Tehran's market and their DNA were extracted by a kit. All samples were analyzed to screen the 35 S promoter and (nopaline synthase) NOS terminator elements. Having screened the GM positive samples, they were subjected to identification of specific transgenic events RR soy, Bt11 and MON810 maize with PCR. According to our results, 95% of soy and 60% of maize analyzed samples were positive for the 35 S promoter and NOS terminator. Furthermore, event-specific analysis indicated the presence of RR soy, Bt11 and MON810 maize in tested samples, while none of these food-derived samples demonstrated any GM label. The obtained results revealed that the necessity of monitoring system to provide a good reliable control of GM materials in food products and subsequently on their labeling.
Thematic areas
Information on Organisms or LMOs
Organism(s) identification
Zea mays - Maize, Corn, MAIZE
Glycine max - Soybean, Soya bean, Soya, SOYBN
Gene(s) identification
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
LMO(s) identification
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
DOI: 10.1080/10942912.2020.1734613
Publisher and its location
International Journal of Food Properties
Taylor & Francis Group
PDF - 1.57 MB (12 pages)
Journal article
Keywords and any other relevant information
PCR; soy; maize; food; electrophoresis