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Information Resource
Record information and status
Record ID
115965
Status
Published
Date of creation
2021-04-07 20:12 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-04-07 20:12 UTC (austein.mcloughlin@cbd.int)

General Information
Title
Capacity building for genetically modified organism (GMO) detection in West Africa: Identifying a circulating GMO maize variety in Mali
Author
Ibrahim Traoré, Diariatou Diagne, Ibrahim Keita, Hinda Doucouré, Mouhamadou Traoré, Niaba Témé, Mamadou Mory Coulibaly, Ousmane A. Koita
Author’s contact information
Ousmane A. Koita
Kindly refer to article for contact information
Language(s)
  • English
Publication date
2019-05-10
Subject
Summary, abstract or table of contents
DNA-based methodology employing quantitative polymerase chain reaction (qPCR) has been successfully used to examine the incidence of genetically modified (GM) maize in Mali. This study aims to ascertain whether screening elements could also be used to detect GM maize. Fourteen maize varieties and one unknown dark color seeded variety from Mali were tested. DNA was extracted from three seeds of each variety. Three screening elements were used for qPCR amplification, the 35S promoter of the Cauliflower mosaic virus (CaMV), the nopaline synthase (NOS terminator) from Agrobacterium tumefaciens and the 35S promoter from the Figwort mosaic virus (FMV). The 14 varieties were negative for P35S CaMV (forward) and T-NOS (reverse) markers. In contrast, the unknown dark color seeded variety was positive with 94 bp PCR product. While, no DNA fragments were amplified using the FMV as the screening element. These data were supported by Ct values in which the 14 varieties had values above 50; whereas, the unknown variety showed values of 24.5 for P-35S-CaMV and 30 for the T-NOS. The study demonstrates the ability in detecting GM maize using screening elements and the usefulness of our laboratory in training and reinforcing regional concern about GMO circulation.
Thematic areas
  • Capacity building
Information on Organisms or LMOs
Organism(s) identification
Zea mays - Maize, Corn, MAIZE
Gene(s) identification
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium
FMV 35S promoter - Figwort mosaic virus - Figwort mottle virus, FMV, CMoVb
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
DOI: 10.5897/AJB2019.16781
Publisher and its location
African Journal of Biotechnology
Academic Journals
Format
PDF - 244 KB (5 pages)
Keywords and any other relevant information
Africa; maize; DNA; PCR; LMO; capacity building