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Modified Organism
Drought tolerant sugarcane
Record information and status
Record ID
116035
Status
Published
Date of creation
2021-05-03 16:15 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-05-03 16:15 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Drought tolerant sugarcane
Transformation event
NXI-4T
Developer(s)
PT Perkebunan Nusantara XI (Persero)
Surabaya
Indonesia, 60175
Phone:+62 031 3524596
Email:sekretariat@ptpn11.co.id
Url:PT Perkebunan Nusantara XI
Description
The sugarcane (Saccharum officinarum) was modified for abiotic (drought, salt) stress tolerance through the action of choline dehydrogenase, which leads to increased glycine betaine biosynthesis. Glycine betaine maintains cell water potential by osmotic adjustment. The expression of choline dehydrogenase may also increase sugar content and promote early maturing.

The sugarcane also contains Escherichia coli neomycin phosphotransferase II and hygromycin B phosphotransferase for kanamycin and hygromycin resistance, respectively.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Saccharum officinarum L. - Sugarcane, Sugar cane
Point of collection or acquisition of the recipient organism
Sugarcane cultivar BL579
Related LMOs
Drought tolerant sugarcane
Changes in physiology and/or production - Yield, Growth rate, Ripening Resistance to antibiotics - Hygromycin, Kanamycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought, Salinity
Characteristics of the transformation process
Vector
pMLH2113
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Omega 5' untranslated leader
0.00 Kb
 
 
Glutamate dehydrogenase mitochondrial transit peptide
0.00 Kb
 
 
Choline dehydrogenase
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified sugarcane contains three gene cassettes: Rhizobium meliloti choline dehydrogenase (betA); Escherichia coli neomycin phosphotransferase (nptII) and E. coli hygromycin B phosphotransferae (hph).

The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a Tobacco mosaic virus omega 5' leader to enhance translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.

The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.

The nptII coding sequence is under transcriptional control of a nos promoter and terminator.

Important notes:
- The donor organism for  the betA is not clear. R. meliloti (one of the suggested donors) was selected based on the attached literature.
- The order of the genetic cassettes is unclear and could be in a different order. However, the genetic elements within each cassette are likely correct.
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to
-90.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Biofuel

Records referencing this document (1)
IDDescription
1record(s) found
Modified Organism1 record