Drought tolerant sugarcane | BCH-LMO-SCBD-116038 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 03 May 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Drought tolerant sugarcane
EN
NXI-1T
No
The sugarcane (Saccharum officinarum) was modified for abiotic (drought, salt) stress tolerance through the action of choline dehydrogenase, which leads to increased glycine betaine biosynthesis. Glycine betaine maintains cell water potential by osmotic adjustment. The expression of choline dehydrogenase may also increase sugar content and promote early maturing.

The sugarcane also contains Escherichia coli neomycin phosphotransferase II and hygromycin B phosphotransferase for kanamycin and hygromycin resistance, respectively.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Sugarcane cultivar CF1426
EN
  • Drought tolerant sugarcane
    | Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
Characteristics of the modification process
pMLH2113
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-116036-1 Glutamate dehydrogenase mitochondrial transit peptide | Solanum lycopersicum (Tomato, SOLLC)
    Transit signal
  • BCH-GENE-SCBD-116037-1 Choline dehydrogenase | Escherichia coli (ECOLX)
    Protein coding sequence | Changes in physiology and/or production (Growth rate, Ripening, Yield),Tolerance to abiotic stress (Drought, Salinity)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)
    Leader
  • BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)
    Leader
The modified sugarcane contains three gene cassettes: Escherichia coli choline dehydrogenase (betA); E. coli neomycin phosphotransferase (nptII) and E. coli hygromycin B phosphotransferae (hph).

The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a 5' leader sequence from Tobacco mosaic virus for enhanced translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.

The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.

The nptII coding sequence is under transcriptional control of a nos promoter and terminator.

Important notes:
- The donor organism for  the betA is not clear. E. coli (one of the suggested donors) was chosen as a placeholder.
- The order of the genetic cassettes is unclear and could be in a different order. However, the genetic elements within each cassette are likely correct.
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to
-90 (E12 ; enhancer).
EN
LMO characteristics
EN
  • Food
  • Biofuel
Detection method(s)
EN
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