MON-ØØ6Ø3-6 × ACS-ZMØØ3-2 × DAS-4Ø278-9 - Herbicide tolerant maize | BCH-LMO-SCBD-116233 | Living Modified Organism | Biosafety Clearing-House

Loading...
Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
last updated: 06 Aug 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant maize
EN
NK603 × T25 × 40278
Yes
MON-ØØ6Ø3-6 × ACS-ZMØØ3-2 × DAS-4Ø278-9
The maize was produced through the cross breeding of modified parental varieties for multiple herbicide tolerance. For glyphosate tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which encodes a variant of an endogenous enzyme involved in the essential biosynthesis of aromatic amino acids (shikimate pathway). The variant prevents the binding of the compound to the enzyme and the subsequent inactivation. For glufinosate tolerance, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates phosphinothricin (the active ingredient in glufosinate ammonium herbicides) through acetylation. Additionally, the modified maize expresses Sphingobium herbicidovorans aryloxyalkanoate dioxygenase, which cleaves 2,4-dichlorophenoxyacetic acid into non-herbicidal dichlorophenol and glyoxylate, as well as inactivates aryloxyphenoxypropionate herbicides (acetyl‐CoA carboxylase inhibitors).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-14776-17 Living Modified Organism MON-ØØ6Ø3-6 - Roundup Ready™ maize
    Monsanto | Resistance to herbicides (Glyphosate)
  • BCH-LMO-SCBD-14767-14 Living Modified Organism ACS-ZMØØ3-2 - Liberty Link™ maize
    Bayer CropScience | Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-104814-1 Living Modified Organism DAS-4Ø278-9 - Enlist™ Maize
    Dow AgroSciences GmbH | Resistance to herbicides, Tolerance to 2,4-Dichlorophenoxyacetic acid, Tolerance to aryloxyphenoxypropionate
EN
Characteristics of the modification process
PV-ZMGT32; pDH51; pDAS1740
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from NK603 vector PV-ZMGT32
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the cp4-epsps. In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and a nos terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by an CaMV enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the maize heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promoter high levels of transcription.

Note:
- The parental NK603 line contained a single, intact insertion containing both cp4-epsps gene cassettes.
- Due to restriction digest prior to particle bombardment, the vector backbone, containing E. coli neomycin phosphotransferase II and origin of replication, were not incorporated into the parental genome.


DNA insert from T25 vector pDH51
The parental genome contains a Streptomyces viridochromogene phosphinothricin N-acetyltransferase gene (pat) gene cassette. Transcription of pat is under control of a Cauliflower mosaic virus 35S promoter and 35S terminator. Expression of pat is therefore expected to occur at elevated levels.

The inserted DNA additionally contains a fragment of the bacterial Escherichia coli beta-lactamase gene and the plasmid vector origin of replication (for bacterial cells). These are not anticipated to be active in plant cells.

Note:
- The pat coding sequence was codon optimized for expression in plants without resulting in amino acid changes in the translated protein.
- A single pat cassette is present in the inserted DNA.


DNA insert from DAS40278 vector pDAS1740
The LMO was generated using the Whiskers-mediated transformation method. Sphingobium herbicidovorans aryloxyalkanoate dioxygenase-1 (aad-1) is under the control of Zea mays ubiquitin gene promoter and Z. mays root preferential cationic peroxidase terminator. Elevated levels of transcription are expected to occur due to the constitutive nature of the ubiquitin promoter.

Note:
- The aad-1 coding sequence was optimized for expression in the plant.
- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus
- No integration of the vector backbone occurred.


Kindly refer to the parental LMO records for more information.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 3
Risk Assessment generated by a regulatory process Living modified organism(s) 3