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Modified Organism
MON-ØØ6Ø3-6 × ACS-ZMØØ3-2 × DAS-4Ø278-9 - Herbicide tolerant maize
Record information and status
Record ID
116233
Status
Published
Date of creation
2021-08-06 19:13 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-08-06 19:13 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant maize
Transformation event
NK603 × T25 × 40278
Unique identifier
MON-ØØ6Ø3-6 × ACS-ZMØØ3-2 × DAS-4Ø278-9
Developer(s)
Pioneer Hi-Bred International Inc.
7100 NW 62nd Avenue
PO Box 1000
Johnston, Iowa
United States of America, 50131
Phone:+1 515 535-3200
Url:Pioneer HiBred International Homepage
Description
The maize was produced through the cross breeding of modified parental varieties for multiple herbicide tolerance. For glyphosate tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which encodes a variant of an endogenous enzyme involved in the essential biosynthesis of aromatic amino acids (shikimate pathway). The variant prevents the binding of the compound to the enzyme and the subsequent inactivation. For glufinosate tolerance, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates phosphinothricin (the active ingredient in glufosinate ammonium herbicides) through acetylation. Additionally, the modified maize expresses Sphingobium herbicidovorans aryloxyalkanoate dioxygenase, which cleaves 2,4-dichlorophenoxyacetic acid into non-herbicidal dichlorophenol and glyoxylate, as well as inactivates aryloxyphenoxypropionate herbicides (acetyl‐CoA carboxylase inhibitors).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
ACS-ZMØØ3-2 - Liberty Link™ maize
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
Show detection method(s)
DAS-4Ø278-9 - Enlist™ Maize
Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMGT32; pDH51; pDAS1740
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
Beta-lactamase gene
0.86 Kb
 
 
pUC origin of replication
2.63 Kb
 
 
CaMV 35S promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.53 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
RB7 matrix attachment region
1.17 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Aryloxyalkanoate dioxygenase gene
0.89 Kb
 
 
Per5 3' Untranslated Region
0.37 Kb
 
 
RB7 matrix attachment region
1.17 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from NK603 vector PV-ZMGT32
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the cp4-epsps. In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and a nos terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by an CaMV enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the maize heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promoter high levels of transcription.

Note:
- The parental NK603 line contained a single, intact insertion containing both cp4-epsps gene cassettes.
- Due to restriction digest prior to particle bombardment, the vector backbone, containing E. coli neomycin phosphotransferase II and origin of replication, were not incorporated into the parental genome.


DNA insert from T25 vector pDH51
The parental genome contains a Streptomyces viridochromogene phosphinothricin N-acetyltransferase gene (pat) gene cassette. Transcription of pat is under control of a Cauliflower mosaic virus 35S promoter and 35S terminator. Expression of pat is therefore expected to occur at elevated levels.

The inserted DNA additionally contains a fragment of the bacterial Escherichia coli beta-lactamase gene and the plasmid vector origin of replication (for bacterial cells). These are not anticipated to be active in plant cells.

Note:
- The pat coding sequence was codon optimized for expression in plants without resulting in amino acid changes in the translated protein.
- A single pat cassette is present in the inserted DNA.


DNA insert from DAS40278 vector pDAS1740
The LMO was generated using the Whiskers-mediated transformation method. Sphingobium herbicidovorans aryloxyalkanoate dioxygenase-1 (aad-1) is under the control of Zea mays ubiquitin gene promoter and Z. mays root preferential cationic peroxidase terminator. Elevated levels of transcription are expected to occur due to the constitutive nature of the ubiquitin promoter.

Note:
- The aad-1 coding sequence was optimized for expression in the plant.
- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus
- No integration of the vector backbone occurred.


Kindly refer to the parental LMO records for more information.
LMO characteristics
Modified traits
  • Tolerance to 2,4-dichlorophenoxyacetic acid
  • Tolerance to aryloxyphenoxypropionate
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record