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Modified Organism
MON-8746Ø-4 × MON-ØØ6Ø3-6 - Herbicide-tolerant, drought-tolerant maize
Record information and status
Record ID
116258
Status
Published
Date of creation
2021-08-25 15:17 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-08-25 15:17 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant, drought-tolerant maize
Transformation event
MON 87460 × NK603
Unique identifier
MON-8746Ø-4 × MON-ØØ6Ø3-6
Developer(s)
Bayer CropScience
Bayer CropScience Deutschland GmbH
Bayer CropScience AG
Alfred-Nobel-Str. 50
40789 Monheim am Rhein
Monheim am Rhein
Germany, 40789
Phone:+49 21 73 - 38-0
Url:Bayer CropScience
Description
The modified maize (Zea mays) was produced through the cross breeding of modified parental lines for herbicide tolerance and drought tolerance. For herbicide tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, a variant of an endogenous enzyme that confers for glyphosate tolerance and allows for continued aromatic amino acid biosynthesis. For drought tolerance, the maize expresses Bacillus subtilis cold shock protein, which binds RNA and maintains cellular functions under water-limited conditions (improvement of natural abiotic stress responses). The maize also contains an Escherichia coli neomycin phosphotransferase II cassette, which allowed for kanamycin selection during transformation of a parental line.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-8746Ø-4 - Droughtgard™ Maize
Resistance to antibiotics - Kanamycin Tolerance to abiotic stress - Cold / Heat, Drought
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMAP595; PV-ZMGT32
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ti plasmid left border repeat
0.36 Kb
 
 
Rice actin 1 gene promoter
0.92 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cold shock protein gene
0.20 Kb
 
 
Transcript 7 gene 3' untranslated region
0.51 Kb
 
 
loxP recombination site
0.03 Kb
 
 
CaMV 35S promoter
0.29 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
loxP recombination site
0.03 Kb
 
 
Ti plasmid right border repeat
0.44 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON87460 vector PV-ZMAP595
The T-DNA insert contains the following gene cassettes: Bacillius subtillus cold shock protein (cspB) and Escherichia coli neomycin phosphotransferase II (nptII). 

Transcription of cspB is under control of the Oryza sativa actin 1 promoter and Agrobacterium tumefaciens transcript 7 gene 3' untranslated region. The transcript initially contains an O. sativa actin 1 intron for enhanced gene expression of cspB. The sequence is removed (spliced) prior to protein translation. Constitutive expression of cspB is expected due to the actin promoter.

Transcription of nptII is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and A. tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the CaMV promoter.

Note:
- The coding sequence of cspB has been codon optimized for optimal expression within plant cells.
- Southern blot analysis indicated that no vector backbone sequences were inserted into the parental genome
- Southern blot analysis indicated that the parental genome contains a single insertion
- Sequencing analyses confirm the Southern blot analyses.
- A 22 base pair deletion of genomic DNA at the insert-to-plant DNA junction occurred.
- loxP sites can be found in the parental genome and could potentially allow for the excision of the nptII cassette by CRE recombinase.


DNA insert from NK603 vector PV-ZMGT32
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the cp4-epsps. In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and a nos terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by an CaMV enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the maize heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promoter high levels of transcription.

Note:
- The parental NK603 line contained a single, intact insertion containing both cp4-epsps gene cassettes.
- Due to restriction digest prior to particle bombardment, the vector backbone, containing E. coli neomycin phosphotransferase II and origin of replication, were not incorporated into the parental genome.


Kindly refer to the parental LMO records for more information.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record