DNA insert from pTHW107 from MS8 (ACS-BNØØ5-8) canola
The DNA insert contains two cassettes: Bacillus amyloliquefaciens barnase and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

Barnase is under control of a Nicotiana tabacum TA29 pollen specific promoter and an Agrobacterium tumefaciens nopaline synthase terminator. An additional sequence, B. amyloliquefaciens barnase 3’ untranslated region, which contributes to the polyadenylation of the coding sequence, can be found between the barnase coding sequence and nopaline synthase terminator.

Phosphinothricin N-acetyltransferase is under control of an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit promoter and A. tumefaciens transcript 7 3’ untranslated region.

Note:
- The coding sequence of bar has the two N-terminal codons modified to ATG and GAC.
- Southern blot and PCR analysis indicated that a single intact copy of the transformation cassette was integrated into the parental genome.


DNA insert from PV-BNGT04 from RT73 (MON-ØØØ73-7) canola
The DNA insert contains two gene cassettes: Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) and Ochrobactrum anthropic glyphosate oxidoreductase (gox).

The cp4-epsps coding sequence is under control of a Figwort mosaic virus 34S promoter and a Pisum sativum rubisco small subunit terminator. A transit peptide from ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit was included before the cp4-epsps coding sequence. The transit peptide leads to high-level of expression in leaf tissues.

Note:
- The size of the genetic elements were not available at time of this record's creation
- The gox coding sequence differs from the wild-type version of the gene at 3 amino acid sites (G85S, R153K and R334H) and was designated as goxv247.
- The cp4-epsps sequence was optimized for expression in plants.
- PCR and southern blot analyses indicated that the parental genome contains a single insertion event containing one copy of the T-DNA from plasmid PV-BNGT04. No genetic elements from outside of the right and left borders of the plasmid were transferred into or are present in the genomic DNA of the LMO.


For more information, kindly refer to the parental LMO records.