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Modified Organism
DAS-Ø15Ø7-1 × MON-87411-9 × DAS-59122-7 - Herbicide-tolerant, insect-resistant maize
Record information and status
Record ID
116321
Status
Published
Date of creation
2021-10-07 19:32 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-10-07 19:32 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant, insect-resistant maize
Transformation event
TC1507 × MON87411 × 59122
Unique identifier
DAS-Ø15Ø7-1 × MON-87411-9 × DAS-59122-7
Developer(s)
Dow AgroSciences GmbH
Dow AgroSciences GmbH
Truderinger Straße 15
München, Bayern
Germany, 81677
Phone:+ 49 89 - 4 55 33 - 0
Fax:+ 49 89 - 4 55 33 - 111
Email:DowAgroSciencesD@dow.com
Url:http://www.dowagro.com/de
Bayer CropScience
Bayer CropScience Deutschland GmbH
Bayer CropScience AG
Alfred-Nobel-Str. 50
40789 Monheim am Rhein
Monheim am Rhein
Germany, 40789
Phone:+49 21 73 - 38-0
Url:Bayer CropScience
Description
The maize (Zea mays) was produced through cross breeding of modified parental maize lines for herbicide tolerance and insect resistance. For herbicide tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (glyphosate tolerance - enzyme variant) and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (glufosinate tolerance - enzymatic inactivation). For Lepidoptera tolerance, the maize expresses Bacillus thuringiensis Cry1F. For Coleoptera resistance, the maize expresses B. thuringiensis Cry3Bb1, Cry34Ab1 and Cry35Ab1. The maize contains an RNA interference cassette targeting Diabrotica virgifera virgifera Snf7 for specific resistance against D. virgifera virgifera.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-87411-9 - Maize modified for herbicide tolerance and insect resistance
Monsanto Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Resistance to herbicides - Glyphosate
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
PHI8999A; PV-ZMIR10871; PHP17662
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.00 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Snf7 coding sequence
0.24 Kb
 
 
Snf7 coding sequence
0.24 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
pIIG gene promoter
0.95 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Heat shock protein 17.3 terminator
0.21 Kb
 
 
Alpha Tubulin Gene promoter
2.18 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Alpha Tubulin Gene terminator
0.58 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Peroxidase gene promoter
1.30 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from TC1507 vector PHI8999A
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for Bacillus thuringiensis cry1F and Streptomyces viridochromogenes pat.

Transcription of cry1F is directed by the promoter and first exon and intron of the maize (Zea mays) ubiquitin gene and terminates at the Agrobacterium tumefaciens ORF25 terminator.

Transcription of the pat gene commences from the Cauliflower mosaic virus (CaMV) 35S promoter and ends at the CaMV 35S terminator.

Note:
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat are identical to those in the original plasmid.
- The CRY1F protein includes the F604K (phenylalanine to lysine at position 604) amino acid substitution, which was introduced to create a specific restriction site for cloning purposes.

DNA insert from MON87411 vector PV-ZMIR10871
The MON87411 genome contains three cassettes: an RNA interference (RNAi) cassette targeting Diabrotica virgifera virgifera, Bacillus thuringiensis cry3Bb1 and Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps).

Transcription of the RNAi cassette commences from the Cauliflower mosaic virus 35S enhanced promoter and terminates at the Pisum sativum ribulose bisphosphate carboxylase small chain 2 terminator. The transcript initially contains a Zea mays heat shock protein 70 intron, which contributes to enhanced expression in vegetative tissues of the plant, and two partial coding sequences of the D. virgifera virgifera Snf7p gene, which encodes the SNF7 subunit of the ESCRT-III complex. The two Snf7p sequences are in an inverted orientation, separated by a 150-nucleotide intervening sequence, which allows base pairing between the inverted sequences and hairpin RNA formation post-transcription, which then triggers an RNAi response. Due to RNAi processing, small interfering RNA molecules (roughly 21-23 nucleotides in length) will be produced and thus no translation into protein will occur from this cassette.

Transcription of the cry3Bb1 is under control of the Z. mays physical impedance induced protein promoter and Triticum aestivum (wheat) heat shock protein 17.3 terminator. The transcript also contains a wheat 5' untranslated leader from chlorophyll a/b-binding protein and Oryza sativa actin 1 intron for enhanced expression of the transgene. Expression of cp4-epsps is under control of an O. sativa alpha tubulin promoter and terminator. The transcript additionally contains Arabidopsis thaliana chloroplast targeting peptide 2 to sequester the protein to the chloroplast.

Note:
- Sequencing, PCR and bioinformatic analyses indicate that a single, intact insertions of the three gene cassettes occurred in the parental line.
- No plasmid backbone was detected.

DNA insert from 59122 vector PHP17662:
Transcription of Bacillus thuringiensis cry34Ab1 starts at Zea mays ubiquitin gene promoter and terminates at the Solanum tuberosum proteinase inhibitor II gene terminator. Transcription of B. thuringiensis cry35Ab1 commences from the (Triticum aestivum (wheat) peroxidase gene promoter and stops at another S. tuberosum proteinase inhibitor II gene terminator.

Note:
- The coding sequence of cry34Ab1 and cry35Ab1 has been adapted to the codon usage in maize as to achieve optimal expression in planta.
- The cry34Ab1 and cry35Ab1 were cloned from B. thuringiensis strain PS149B1.
- Sequence analysis of 59122 done by the European Food Safety Authority indicated that this LMO contains one complete copy of the T-DNA of PHP17662 without internal rearrangements. All three gene cassettes, cry34Ab1, cry35Ab1 and pat, are intact within the transgenic event. The DNA sequences of the genes in 59122 are identical to those in the original plasmid except for two nucleotide differences in the wheat peroxidase promoter. At the 5' T-DNA end a deletion of 22 bp is observed and at the 3' T-DNA end a deletion of 25 bp is observed. The absence of vector backbone in maize 59122 was also demonstrated.

For more information, kindly refer to the parental LMO records.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
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2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record