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Living Modified Organism (LMO)
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Line Oxy-235 was developed to allow the use of oxynil herbicides, ioxynil and bromoxynil, as weed control options in canola. Canola tolerant to the oxynil herbicides created through insertion of the bxn gene isolated from the bacterium Klebsiella ozaenae, encoding a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds. Bromoxynil is very effective on broadleaf weeds that are common in canola fields and the deployment of transgenic bromoxynil-tolerant canola will allow the post-emergence control of these weeds without crop injury.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Canola variety Westar
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
BCH-GENE-SCBD-14976-5 Bromoxynil-specific nitrilaseProtein coding sequence | Resistance to herbicides (Bromoxynil)
BCH-GENE-SCBD-100287-7 CaMV 35S promoterPromoter
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene TerminatorTerminator
BCH-GENE-SCBD-103768-2 RuBisCO small subunit gene 5'UTR | (Maize, Corn)Leader
Transgenic Oxy-235 contains a single copy of the bxn gene isolated from the bacterium Klebsiella pneumoniae (subsp. ozaenae), which encodes a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds. Constitutive expression of the bxn gene was regulated via the 35S promoter from cauliflower mosaic virus (CaMV) and levels of nitrilase enzyme were detected in samples of leaf (1000 ng/mg total protein) and seed (<10 ng/mg total protein) tissue. No nitrilase was detectable in samples of refined oil (detection limit 20 ppb), and it was concluded that there will be no human exposure to this proteins.