Record details

Living Modified Organism identity

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LMO name

High oleic acid soybean

Transformation event

260-05 (Lines: G94-1, G94-19, G-168)

Unique identifier

DD-Ø26ØØ5-3

Developer(s)

Record #14931

Pioneer Hi-Bred International Inc.

7100 NW 62nd Avenue
PO Box 1000

Johnston, Iowa

United States of America, 50131

Phone:	+1 515 535-3200
Url:	Pioneer HiBred International Homepage

Record #4944

DuPont

Url:	DuPont Homepage

Description

Soybean lines G94-1, G94-19 and G-168 were developed through a specific genetic modification to produce a soybean oil that contains high levels of oleic acid, a monounsaturated fatty acid. These high oleic soybeans contain a second copy of fatty acid desaturase gene (fad2), which is naturally present in soybeans. The fad2 gene codes for the enzyme, delta-12 desaturase, which is involved in fatty acid synthesis. Unlike conventional soybeans, the presence of a second copy of the fad2 gene in the high oleic soybeans G94-1, G94-19 and G-168 causes a phenomenon known as "sense suppression" which results in both copies of the fatty acid desaturase gene being "switched off". This blocks the fatty acid biosynthetic pathway and results in the accumulation of oleic acid. As a consequence, polyunsaturated fatty acids (linoleic acid and linolenic acid) are only produced in very small amounts.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #10453

Glycine max - Soybean, Soya bean, Soya, SOYBN

Characteristics of the transformation process

Vector

pBS43 and pML102

Techniques used for the modification

Biolistic / Particle gun

Genetic elements construct

α' subunit of β-conglycinin gene promoter

#104359

0.61 Kb

delta(12)-fatty acid dehydrogenase

#100267

1.46 Kb

Phaseolin 3' Terminator

#104364

1.17 Kb

CaMV 35S promoter

#100287

1.40 Kb

5' untranslated leader of chlorophyll a/b-binding protein

#101901

0.06 Kb

Beta-glucuronidase coding sequence

#46004

1.85 Kb

Nopaline Synthase Gene Terminator

#100269

0.77 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

High oleic acid soybean produced by inserting a second copy of the fatty acid desaturase (GmFad2-1) gene from soybean, which resulted in "silencing" of the endogenous host gene.

The transgenic fad2 gene in G94-1, G94-19 and G-168 was isolated from Glycine max (soybean) and introduced into a commercial soybean variety using particle acceleration (biolistic) transformation. The genetic modification affects only the seed, allowing fatty acid biosynthesis to function normally in other plant parts such as the leaves.

The original transformant was co-bombarded with vector pML102 which contained the dapA gene from the bacterium Corynebacterium glutanicum, encoding dihydrodipicolinic acid synthase, expression of which can lead to an increase in free lysine content. The dapA gene was inserted independently from the GmFad2-1 gene and was lost via segregation, and is thus not present in the subject soybean lines.

LMO characteristics

Modified traits

Changes in quality and/or metabolite content
- Lipid and fatty acids
Production of medical or pharmaceutical compounds (human or animal)
- Omega-3 fatty acids (e.g. DHA)

Common use(s)

Food
Feed

Additional Information

High oleic soybean oil contains levels of oleic acid exceeding 80%, higher than the levels found in olive oil and rapeseed oil. This oil is lower in saturated fat, contains no trans-fatty acids, and remains in a liquid form. The high levels of oleic acid make the oil more heat-stable for cooking and edible spray applications.

Other relevant website address or attached documents

OECD UID Database

CERA GM Database

High oleic acid soybean APHIS.pdf