FLO-11363-2 - Moonshadow™ carnation | BCH-LMO-SCBD-14772 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 26 Jan 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moonshadow™ carnation
EN
11363 (1363A)
FLO-11363-2
Carnation Moonshadow 1363A has a modified flower colour, a shade of light mauve, whereas the non-GM parent has cream-white flowers. The colour has been achieved by introducing into white carnation two genes of the anthocyanin biosynthesis pathway from Petunia and Viola sp. These genes, encoding dihydroflavonol 4-reductase (dfr) and flavonoid 3'5' hydroxylase (f3'5'h), together with other genes of the anthocyanin biosynthesis pathway already present in the non GM carnation, give rise to the anthocyanins delphinidin and cyanidin.

Note: No longer available, was sold for about 10 years, has been replaced by superior varieties

NOTE: This LMO was formerly referred to with the UID FLO-11363-1.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: White Unesco
EN
  • FLO-4Ø689-6 - Moonaqua™ carnation
    | Dr Yoshikazu Tanaka Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
  • FLO-11226-9 - Moonshade™ carnation
    | Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
  • FLO-4Ø685-2 - Moonvista™ carnation
    | Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
  • FLO-11351-8 - Moonshade™ carnation
    | Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
  • FLO-114ØØ-3 - Moonshade™ carnation
    | Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
  • FLO-11959-4 - Moonshade™ carnation
    | Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
  • FLO-11988-6 - Moonshade™ carnation
    | Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the modification process
pCGP1991
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Southern blot analysis indicated that there are 4 to 6 copies of the insert present in FLO-11363-1. Three integration sites were sequenced, each of which consist of both complete and partial T-DNA sequences. Locus 1 consists of three parts of the T-DNA of which two parts are in a sense orientation and one in anti-sense orientation. Locus 2 consists of a partial T-DNA in the anti-sense orientation and one complete T-DNA in the sense orientation. Locus 3 consists of two complete T-DNA in the sense orientation and a partial T-DNA in the anti-sense orientation. 

Southern blot analysis also indicated the absence of vector backbone sequences.
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
Three genes have been transferred into FLO-4Ø689-6, these are:

• The petunia DFR gene, coding for dihydroflavonol 4-reductase (DFR), derived from Petunia X hybrida. The petunia DFR enzyme is only capable of using dihydroquercetin and dihydromyricetin as substrate, not dihydrokaempferol. This ensures that most or all of the anthocyanidin produced is delphinidin. A constitutive promoter drives the petunia DFR-A cDNA derived gene;

• the pansy F3'5'H gene, coding for flavonoid 3' 5' hydroxylase (F3'5'H), derived from Viola sp. F3'5'H acts by converting the dihydroflavonols dihydrokaempferol and/or dihydroquercetin into the dihydroflavonol dihydromyricetin. The cDNA for F3'5'H encodes the enzyme F3'5'H allowing transgenic plants normally lacking this enzyme to produce violet or blue delphinidin derived pigments; and

• ALS gene (SuRB), coding for a mutant acetolactate synthase protein (ALS), derived from Nicotiana tabacum. Expression of the mutation confers resistance to sulfonylurea herbicides.
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 3
Country's Decision or any other Communication Living modified organism(s) 3
Living Modified Organism Related LMO(s) 8